瑞芬太尼调节IL-10/β-内啡肽信号通路对坐骨神经损伤大鼠疼痛的影响  

Effect of remifentanil on pain behavior in rats with sciatic nerve injury by regulating the IL-10/β-endorphin signaling pathway

作　　者：封雪[1] 赵滨滨[1] 任蓁[2] FENG Xue;ZHAO Binbin;REN Zhen(Department of Anesthesiology and Surgery,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Heilongjiang,Harbin 150040,China;不详)

机构地区：[1]黑龙江中医药大学附属第一医院麻醉手术科,哈尔滨市150040 [2]黑龙江中医药大学附属第一医院护理教研室,哈尔滨市150040

出　　处：《河北医药》2024年第7期988-992,共5页Hebei Medical Journal

基　　金：黑龙江省卫生健康委科研课题(编号:20220404110755)。

摘　　要：目的探讨瑞芬太尼对坐骨神经损伤(SNI)大鼠疼痛的影响及作用机制。方法建立SNI大鼠模型,大鼠分为假手术组、模型组、瑞芬太尼低剂量组(5μg·kg^(-1)·min^(-1))、瑞芬太尼高剂量组(20μg·kg^(-1)·min^(-1))和瑞芬太尼+抑制剂组(20μg·kg^(-1)·min^(-1)的瑞芬太尼+10μL IL-10抗体),评估大鼠疼痛阈值,统计坐骨神经指数(SFI),Masson染液评价靶肌肉萎缩情况,酶联免疫吸附法(ELISA)检测白介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α的含量,免疫荧光检测微管相关蛋白(MAP2)的表达,Western blot检测IL-10、β-内啡肽蛋白表达。结果与假手术组比较,模型组大鼠疼痛阈值、SFI和IL-10、β-内啡肽蛋白的表达水平下降,肌肉萎缩、IL-1β、IL-6、TNF-α、MAP2表达增加(P<0.05);与模型组比较,瑞芬太尼低、高剂量组大鼠疼痛阈值、SFI和IL-10、β-内啡肽蛋白的表达水平升高,肌肉萎缩、IL-1β、IL-6、TNF-α、MAP2表达降低,且呈剂量依赖性(P<0.05);与瑞芬太尼高剂量组比较,瑞芬太尼+抑制剂组大鼠疼痛阈值、SFI和IL-10、β-内啡肽蛋白的表达水平下降,肌肉萎缩、IL-1β、IL-6、TNF-α、MAP2表达增加(P<0.05)。结论瑞芬太尼可能通过激活IL-10/β-内啡肽信号通路抑制SNI大鼠的疼痛行为。Objective To investigate the effect of remifentanil on pain behavior in sciatic nerve injury(SNI)rats and the underlying mechanism.Methods The SNI rat model was established.Rats were randomly divided into sham operation group(cut open without SNI modeling),model group(SNI modeling),low-dose remifentanil group(5μg·kg^(-1)·min^(-1)),high-dose remifentanil group(20μg·kg^(-1)·min^(-1))and remifentanil+inhibitor group(20μg·kg^(-1)·min^(-1) remifentanil+10μL of anti-IL-10 antibody).The pain threshold and sciatic nerve index(SFI)of rats were evaluated.Masson staining was applied to evaluate target muscle atrophy.Enzyme-linked immunosorbent assay(ELISA)was applied to detect the levels of interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α).Immunofluorescence was applied to detect the positive expression of microtubule-associated protein 2(MAP2).Western blot was applied to detect the protein expressions of IL-10 and β-endorphin.Results Compared with those of the sham operation group,rats in the model group presented significantly lower pain threshold,SFI and protein expressions of IL-10 and β-endorphin,muscle atrophy,and significantly higher levels of IL-1β,IL-6 and TNF-α and protein expression of MAP2(P<0.05).Compared with those of model group,rats in the low-dose and high-dose remifentanil groups presented significantly higher pain threshold,SFI and protein expressions of IL-10 and β-endorphin,alleviated muscle atrophy,and significantly lower levels of IL-1β,IL-6 and TNF-α and protein expression of MAP2 in a dose-dependent manner(P<0.05).Compared with those of the high-dose remifentanil group,rats in the remifentanil+inhibitor group presented significantly lower pain threshold,SFI and protein expressions of IL-10 andβ-endorphin,muscle atrophy,and significantly higher levels of IL-1β,IL-6 and TNF-α and protein expression of MAP2(P<0.05).Conclusion Remifentanil may inhibit pain behavior in SNI rats by activating the IL-10/β-endorphin signaling pathway.

关 键 词：瑞芬太尼 坐骨神经损伤 疼痛 白介素-10/β-内啡肽 

分 类 号：R614[医药卫生—麻醉学]