一测多评法同时测定丁公藤不同炮制品中7个活性成分含量  

Simultaneous determination of 7 active ingredients in Erycibes Caulis with different processing technology by QAMS

作　　者：杨海玲 李雲妃[1] 刘振杰 陆星宇 潘忠旭 YANG Hai-ling;LI Yun-fei;LIU Zhen-jie;LU Xing-yu;PAN Zhong-xu(Anhui Medical College,Hefei 230601,China;Guangxi Traditional Chinese Medical University,Nanning 530001,China)

机构地区：[1]安徽医学高等专科学校,合肥230601 [2]广西中医药大学,南宁530001

出　　处：《药物分析杂志》2024年第3期395-404,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(81860711);广西高校中青年教师科研基础能力提升项目(2020KY07034);广西壮瑶药重点实验室(桂科基[2014]32号(20-065-14));广西壮瑶药重点实验室开放课题(GXZYKF2020A-10);广西中医药大学创新训练项目(DXS2019054);2021年广西中医药大学校级科研项目(2021MS004);中药炮制技术传承基地(桂中医药发【2022】9号);2023年国家级大学生创新创业训练计划项目(202310600012)。

摘　　要：目的:建立一测多评法同时测定丁公藤及其炮制品中7个活性成分含量,并分析炮制对其影响。方法:采用超高效液相色谱法(UPLC),以绿原酸为参照物,建立新绿原酸、东莨菪苷、东莨菪内酯、异绿原酸B、异绿原酸A和异绿原酸C的相对校正因子。分别采用外标一点法和一测多评法测定上述7个活性成分含量,比较计算值与实测值的差异,以验证一测多评法的准确性和和可行性。结果:新绿原酸、东莨菪苷、绿原酸、东莨菪内酯、异绿原酸B、异绿原酸A、异绿原酸C分别在0.028~1.420、0.019~0.956、0.027~1.324、0.014~0.720、0.017~0.824、0.010~0.500和0.013~0.672μg·μL^(-1)范围内呈现良好线性关系,平均加样回收率(n=6)分别为98.9%、99.0%、100.6%、101.2%、100.8%、101.7%和100.5%,RSD分别为1.1%、1.7%、1.5%、1.6%、0.55%、1.6%和1.5%。采用校正因子计算的7个活性成分含量值与外标法实测值之间无显著差异。不同丁公藤饮片中新绿原5个有机酸(新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C)含量变化趋势基本一致,Y5(甘草汁-盐水煮品)含量最高,Y10(甘草汁-盐水晒品Ⅱ)含量最低,东莨菪苷含量以Y5含量最高(3.53 mg·g^(-1)),Y10最低(0.31 mg·g^(-1))。东莨菪内酯含量以Y7(甘草汁晒品Ⅰ)含量最高(1.48 mg·g^(-1)),Y3(甘草汁煮品Ⅱ)含量最低(0.30 mg·g^(-1))。结论:以绿原酸为参照物建立的一测多评法可用于丁公藤饮片质量评价,加热和加辅料炮制均对丁公藤的化学成分产生了一定影响。Objective:To establish a method and validate its feasibility for quality evaluation of Erycibes Caulis and its processed products,and analyze the effect on the contents of the seven active ingredient before and after processing.Methods:The contents of components were determined by ultra performance liquid chromatography(UPLC).Chlorogenic acid was chosen as the internal reference substances,the relative correction factors(RCFs)of neochlorogenic acid scopolin,scopoletin,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C and to chlorogenic acid were established.The contents of these seven active ingredients were determined by external standard method and quantitative analysis of multi-components by single-marker(QAMS)method.The method was evaluated by comparison of the quantitative results between external standard method and quantitative analysis of multi-components by single-marker(QAMS).Results:The peaks of neochlorogenic acid,scopolin,chlorogenic acid,scopoletin,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the sample showed good linear relationship between 0.028-1.420μg·μL^(-1),0.019-0.956μg·μL^(-1),0.027-1.324μg·μL^(-1),0.014-0.720μg·μL^(-1),0.017-0.824μg·μL^(-1),0.010-0.500μg·μL^(-1)and 0.013-0.672μg·μL^(-1),respectively.The average recoveries of them(n=6)were as follows 98.9%,99.0%,100.6%,101.2%,100.8%,101.7%and 100.5%,with the RSDs of 1.1%,1.7%,1.5%,1.6%,0.55%,1.6%and 1.5%,respectively.It was showed that no significant difference was found in the quantitative results of seven ingredients by external standard method and QAMS method.The content of 5 kinds of organic acids(neochlorogenic acid,chlorogenic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C)in Erycibes Caulis with different processing technology showed the same change trend.The sample of Y_5(boiled products with licorice sauce and brine)had the highest contents,and the sample of Y_(10)(dried productsⅡsoaked in licorice sauce and brine)had the lowest.The conte

关 键 词：丁公藤 炮制 东莨菪苷 东莨菪内酯 新绿原酸 绿原酸 异绿原酸 一测多评法 

分 类 号：R917[医药卫生—药物分析学]