电化学发光免疫测定技术对桥本氏甲状腺炎的灵敏性、特异性分析  

Sensitivity and Specificity Analysis of Electrochemiluminescence Immunoassay Technology for Hashimoto's Thyroiditis

作　　者：王贵贤[1] 魏旭明[1] 杨明[1] WANG Guixian;WEI Xuming;YANG Ming(Department of Laboratory,Second Affiliated Hospital of Xinxiang Medical College,Xinxiang Henan 453002,China)

机构地区：[1]河南新乡医学院第二附属医院检验科,河南新乡453002

出　　处：《临床研究》2024年第5期24-27,共4页Clinical Research

摘　　要：目的 探讨桥本氏甲状腺炎(HT)患者应用电化学发光免疫测定技术(ECLIA)的灵敏性、特异性。方法 选取2020年8月至2022年8月在河南新乡医学院第二附属医院检查的疑似HT患者共计68例,所有患者均接受ECLIA、放射免疫法(RIA)测定,以症状、病理检查及随访结果作为金标准,比较不同测定技术的测定结果、阳性率、诊断价值及测定时间。结果 ECLIA测定游离四碘甲状腺原氨酸(FT4)、游离三碘甲状腺原氨酸(FT3)、抗甲状腺过氧化物酶抗体(TPOAb)、促甲状腺激素(TSH)、抗甲状腺球蛋白抗体(TgAb)、促甲状腺激素受体抗体(TRAb)水平较RIA更高,差异均有统计学意义(P <0.05);ECLIA测定FT3、FT4、TSH、TPOAb、TgAb、TRAb阳性率较RIA更高,差异均有统计学意义(P <0.05);ECLIA诊断灵敏度、特异度、阳性预测值、阴性预测值、准确性、曲线下面积(AUC)分别为95.83%、95.00%、97.87%、90.48%、95.59%、0.861,RIA分别为79.17%、60.00%、82.61%、54.54%、73.53%、0.753,ECLIA较RIA更高,差异具有统计学意义(P <0.05);ECLIA平均测定时间时间较RIA更短,差异具有统计学意义(P <0.05)。结论 ECLIA用于HT患者灵敏性、特异性均较高,且可缩短测定时间。Objective To explore the sensitivity and specificity of Electrochemiluminescence Immunoassay(ECLIA) in patients with Hashimoto's thyroiditis(HT).Methods A total of 68 suspected HT patients who were examined at the Second Affiliated Hospital of Xinxiang Medical College in Henan from August 2020 to August 2022 were selected.All patients underwent ECLIA and Radioimmunoassay(RIA) tests,with symptoms,pathological examination,and follow-up results as the gold standard.The test results,positive rates,diagnostic value,and test time of different testing techniques were compared.Results The levels of Free Thyroxine(FT4),Free Triiodothyronine(FT3),Thyroid Peroxidase Antibody(TPOAb),Thyroid Stimulating Hormone(TSH),Thyroglobulin Antibody(Tg Ab),and Thyroid Stimulating Hormone Receptor Antibody(TRAb) measured by ECLIA were higher than those by RIA,and the differences were statistically significant(P < 0.05);The positive rates of FT3,FT4,TSH,TPOAb,Tg Ab,and TRAb measured by ECLIA were higher than those by RIA,and the differences were statistically significant(P < 0.05);The sensitivity,specificity,positive predictive value,negative predictive value,accuracy,and area under the curve(AUC) of ECLIA diagnosis were 95.83%,95.00%,97.87%,90.48%,95.59%,and 0.861,respectively,while those of RIA were 79.17%,60.00%,82.61%,54.54%,73.53%,and 0.753,respectively.ECLIA was higher than RIA,and the differences were statistically significant(P < 0.05);The average test time of ECLIA was shorter than that of RIA,and the difference was statistically significant(P <0.05).Conclusion ECLIA has higher sensitivity and specificity in HT patients and can shorten the test time.

关 键 词：桥本氏甲状腺炎 电化学发光免疫测定技术 灵敏性 特异性 

分 类 号：R581.4[医药卫生—内分泌]