丙氨酸氨基转移酶活性在不同保存条件下的稳定性分析  

Stability Analysis of Alanine Aminotransferase Activity under different Preservation Conditions

作　　者：王静 李刚 张莉 WANG Jing;LI Gang;ZHANG Li(Department of Body Collection,Zhumadian Central Blood Station,Zhumadian Henan 463000,China;Department of Inspection,Zhumadian Central Blood Station,Zhumadian Henan 463000,China)

机构地区：[1]驻马店市中心血站体采科,河南驻马店463000 [2]驻马店市中心血站检验科,河南驻马店463000

出　　处：《临床研究》2024年第5期142-144,共3页Clinical Research

摘　　要：目的 分析丙氨酸氨基转移酶(ALT)活性在各种保存条件下的稳定性。方法 将驻马店市中心血站2022年1月至2023年1月ALT快速检验合格的健康献血者血样(266例)作为研究对象,采集健康献血者抗凝血标本,随即立刻检测ALT初始值,完成上述操作后对血浆实施分离,分别在4℃、25℃、37℃条件下保存,分别检测不同时间ALT活性。结果 ALT在4℃存放72 h内测定值差异无统计学意义(P> 0.05);ALT在25℃下放置24 h测定值差异无统计学意义(P> 0.05),48 h测定值开始发生明显变化,差异有统计学意义(P <0.05);37℃温度下保存12 h其检测值开始发生显著性变化,差异有统计学意义(P <0.05)。结论 ALT活性与保存环境内温度密切相关,为确保血ALT检验结果准确无误,建议血样标本采集后24 h完成检验,如不能即刻检验,应分离血清并低温保存。Objective To analyze the stability of alanine aminotransferase(ALT) activity under various storage conditions.Methods A total of 266 healthy blood donors who passed the rapid ALT test at the central blood station in Zhumadian from January 2022 to January 2023 were selected as the research subjects.Anti coagulation samples from healthy blood donors were collected,and the initial ALT value was immediately tested.After completing the above operations,the plasma was separated and stored at 4 ℃,25 ℃,and 37 ℃,and ALT activity was tested at different times.Results There was no statistically significant difference in the measured values of ALT after being stored at 4 ℃ for 72 hours(P > 0.05);There was no statistically significant difference in the measured values of ALT after being placed at 25 ℃ for 24 hours(P > 0.05),but the measured values began to show significant changes after 48 hours,and the difference was statistically significant(P < 0.05);The detection value began to show significant changes after being stored at 37 ℃ for 12 hours,and the difference was statistically significant(P < 0.05).Conclusion ALT activity is closely related to the temperature in the storage environment.To ensure the accuracy of blood ALT test results,it is recommended to complete the test 24 hours after the collection of blood samples.If immediate testing is not possible,serum should be separated and stored at low temperature.

关 键 词：丙氨酸氨基转移酶活性 不同保存条件 温度 稳定性 

分 类 号：R977.3[医药卫生—药品]