去氢骆驼蓬碱对炎症性肠病模型小鼠的防护作用  

Protective Effect of Harmine on Model Mice with Inflammatory Bowel Disease

作　　者：谭燕杰 石郎天 姚勇刚 TAN Yanjie;SHI Langtian;YAO Yonggang(Hunan University of Chinese Medicine,Changsha,Hunan,China 410208;Chongqing Traditional Chinese Medicine Hospital,Chongqing,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]重庆市中医院,重庆400011

出　　处：《中国药业》2024年第8期32-35,共4页China Pharmaceuticals

基　　金：国家自然科学基金[31800983];重庆市自然科学基金面上项目[cstc2019jcyj-msxmX0101]。

摘　　要：目的探讨去氢骆驼蓬碱对炎症性肠病(IBD)模型小鼠肠道屏障的防护作用。方法将64只C57BL/6J雄性小鼠随机分为空白对照组(等体积水)、药物对照组(0.05 mg/mL去氢骆驼蓬碱)、模型组(等体积水)及药物观察组(0.05 mg/mL去氢骆驼蓬碱),各16只。在小鼠饮水中添加2%硫酸葡聚糖钠连续喂养7 d以复制IBD小鼠模型;期间2个对照组小鼠均饮水。建模成功后,各组小鼠连续4 d予相应药物或水。观察小鼠生存率、体质量及结肠长度变化情况。采用苏木素-伊红染色,观察小鼠结肠组织病理改变,并评分。采用Western blot法检测小鼠肠道ZO-1和Occludin蛋白表达水平。结果与模型组比较,药物观察组小鼠生存率明显升高,体质量变化率明显减小,结肠长度明显增加(P<0.05);结肠黏膜和黏膜下层上皮损伤明显减轻,炎性细胞浸润明显减少,且病理学评分明显降低(P<0.05);肠道ZO-1和Occludin蛋白表达水平明显升高(P<0.05)。结论去氢骆驼蓬碱对IBD模型小鼠肠道屏障具有防护作用,其机制可能为提高ZO-1和Occludin蛋白的表达水平。Objective To investigate the protective effect of harmine on the intestinal barrier of model mice with inflammatory bowel disease(IBD).Methods A total of 64 C57BL/6J male mice were randomly divided into the blank control group(equal volume of water),the drug control group(0.05 mg/mL harmine),the model group(equal volume of water),and the drug observation group(0.05 mg/mL harmine),with 16 mice in each group.The mice were given the drinking water containing 2%dextran sulphate sodium for 7 d to replicate the IBD model,while the mice in the blank control group and drug control group were given the water.After successful modeling,the mice in each group were given corresponding drugs or water for 4 d.The survival rate,body mass and colon length of mice were observed.The pathological change of colon tissue was observed by the hematoxylin-eosin staining and scored.The expression levels of ZO-1 and Occludin proteins in the intestine of mice were detected by the Western blot.Results Compared with those in the model group,the survival rate significantly increased,the change rate of body weight significantly decreased,and the colon length significantly increased in the drug observation group(P<0.05);the epithelial injury and inflammatory cell infiltration of colon mucosa and submucosa significantly relieved,and pathological scores in the drug observation group significantly decreased(P<0.05);the expression levels of ZO-1 and Occludin proteins in the intestine of mice in the drug observation group significantly increased(P<0.05).Conclusion Harmine has a protective effect on the intestinal barrier of IBD model mice,and its mechanism may be related to increasing the expression levels of ZO-1 and Occludin proteins.

关 键 词：去氢骆驼蓬碱 炎症性肠病 肠道屏障 小鼠 ZO-1蛋白 OCCLUDIN蛋白 

分 类 号：R965[医药卫生—药理学]