高效液相色谱法同时测定橘红颗粒中3种黄酮类成分含量  

Simultaneous Determination of Three Flavonoids in Juhong Granules by HPLC

作　　者：李霞 易徐航 杨恢检 熊毓 LI Xia;YI Xuhang;YANG Huijian;XIONG Yu(Pingxiang Institute for Food and Drug Control,Pingxiang,Jiangxi,China 337000)

机构地区：[1]江西省萍乡市食品药品检验所,江西萍乡337000

出　　处：《中国药业》2024年第8期79-82,共4页China Pharmaceuticals

摘　　要：目的建立同时测定橘红颗粒中芸香柚皮苷、柚皮苷和橙皮苷含量的高效液相色谱法。方法色谱柱为Agilent 5TC-C18(2)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%醋酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为280 nm,柱温为30℃,进样量为10μL。结果芸香柚皮苷、柚皮苷和橙皮苷进样量分别在0.0348~0.348μg(r=0.9995)、1.047~10.47μg(r=0.9994)、0.1859~1.859μg(r=0.9997)范围内与峰面积线性关系良好;精密度、稳定性、重复性试验结果的RSD均不大于2.0%;平均加样回收率分别为98.24%,96.29%,97.83%,RSD分别为1.16%,1.08%,2.34%(n=6)。结论所建立的方法操作简便、重复性好、结果准确,可用于同时测定橘红颗粒中上述3种黄酮类成分的含量。Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of narirutin,naringin and hesperidin in Juhong Granules.Methods The chromatographic column was Agilent 5TC-C18(2)column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.1%acetic acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 280 nm,the column temperature was 30℃,and the injection volume was 10μL.Results The linear ranges of narirutin,naringin and hesperidin were 0.0348-0.348μg(r=0.9995),1.047-10.47μg(r=0.9994),0.1859-1.859μg(r=0.9997),respectively.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of the above three flavonoids were 98.24%,96.29%,97.83%,with the RSDs of 1.16%,1.08%,2.34%(n=6)respectively.Conclusion The established method is easy,repeatable and accurate,which can be used to simultaneously determine the contents of the above three flavonoids in Juhong Granules.

关 键 词：橘红颗粒 芸香柚皮苷 柚皮苷 橙皮苷 黄酮类成分 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]