妊娠期糖尿病患者血清miR-33 ABCA1表达与病情及妊娠结局的关系  

Relationship between the Expression of Serum miR-33 ABCA1 in Patients with Gestational Diabetes and the Condition and Pregnancy Outcome

作　　者：李红霞 曾欢 LI Hongxia;ZENG Huan(Xiantao First People's Hospital,Hubei Xiantao 433000,China)

机构地区：[1]湖北省仙桃市第一人民医院妇产科,湖北仙桃433000

出　　处：《河北医学》2024年第4期570-575,共6页Hebei Medicine

基　　金：湖北省卫生计生委科研项目(编号:WJ2019S020)。

摘　　要：目的:探讨妊娠期糖尿病患者血清miR-33,ABCA1表达与病情及妊娠结局的关系。方法:回顾性分析2022年4月至2023年4月于本院产科产检并诊断为妊娠期糖尿病的75例孕妇(糖尿病组),并以64例正常妊娠的孕妇为对照组,抽取空腹血样,计算胰岛素抵抗;逆转录-定量实时PCR法测定血清miR-33表达水平;酶联免疫吸附法测定血清ABCA1水平;比较两组及不同Priscilla WHITE分级患者中血清miR-33、ABCA1水平;以Pearson法分析血清miR-33、ABCA1水平相关性以及二者与胰岛素抵抗的相关性;比较两组受试人员及新生儿不良结局;分析根据是否发生不良妊娠结局,将妊娠期糖尿病分为妊娠良好组(42例)、妊娠不良组(33例),比较两组血清ABCA1、miR-33水平;Logistics回归分析影响妊娠期糖尿病患者结局的因素。结果:糖尿病组血清中ABCA1水平较对照组显著降低(t=9.839,P=0.000),miR-33表达、胰岛素抵抗显著增加(t=9.256,P=0.000),在分娩孕周、初次妊娠、体质量以及年龄方面无统计学差异(P>0.05),随着病情加重,miR-33表达水平随之增加(t=5.231,P=0.000),ABCA1水平随之降低(t=9.421,P=0.000)。妊娠期糖尿病患者血清ABCA1与miR-33、胰岛素抵抗具有负相关性(r=-0.685,-0.510,P=0.000),但miR-33与胰岛素抵抗具有正相关性(r=0.535,P=0.000);糖尿病组巨大儿、新生儿低血糖、胎膜早破的发生率显著高于对照组(P<0.05);妊娠不良组血清中miR-33较妊娠良好组显著增加(P<0.05),但ABCA1水平显著降低(P<0.05);血清miR-33、ABCA1水平、胰岛素抵抗为妊娠期糖尿病患者发生不良妊娠的独立影响因素(P<0.05)。结论:妊娠期糖尿病患者血清miR-33显著增加,ABCA1表达降低,均与胰岛素抵抗相关,妊娠结局受血清miR-33、ABCA1水平、胰岛素抵抗等影响。Objective:To investigate the relationship between the expression of serum miR-33 and AB-CA1 in patients with gestational diabetes and the condition and pregnancy outcome.Methods:Retrospective analysis was made on 75 pregnant women(diabetes group)who were diagnosed with gestational diabetes dur-ing obstetric examination in our hospital from April 2022 to April 2023,and 64 pregnant women with normal pregnancy as the control group,fasting blood samples were taken and insulin resistance was calculated;re-verse transcription quantitative real-time PCR method was applied to measure the expression level of serum miR-33;enzyme linked immunosorbent assay(ELISA)was applied to measure serum ABCA1 level;the ser-um levels of miR-33 and ABCA1 were compared between two groups and among patients with different Priscil-la WHITE grades;Pearson method was applied to analyze the correlation between serum miR-33 and ABCA1 levels,and their correlation with insulin resistance;the adverse outcomes of subjects and newborns in two groups were compared;according to the adverse pregnancy outcome,the patients with gestational diabetes were separated into good pregnancy group(42 cases)and poor pregnancy group(33 cases),the serum levels of ABCA1 and miR-33 were compared between the two groups;Logistic regression was applied to analyze the factors affecting the outcome of gestational diabetes.Results:The serum ABCA1 level in the diabetes group was obviously lower than that in the control group(t=9.839,P=0.000),and the expression of miR-33 and insulin resistance were obviously increased(t=9.256,P=0.000),there was no statistical difference in the gestational week,first pregnancy,body mass and age(P>0.05),as the condition worsened,the expression level of miR-33 increased(t=5.231,P=0.000)and the level of ABCA1 decreased(t=9.421,P=0.000).Serum ABCA1 was negatively correlated with miR-33 and insulin resistance in patients with gestational diabetes(r=-0.685,-0.510,P=0.000),but miR-33 was positively correlated with insulin resistance(r=0.535,P=0.000);t

关 键 词：妊娠期糖尿病 miR-33 ABCA1 妊娠结局 

分 类 号：R714.256[医药卫生—妇产科学]