肤黄止痒汤抑制IL-31/TRPV1/ERK1/2信号轴缓解尿毒症小鼠皮肤瘙痒的研究  

The study of Fu Huang Zhi Yang Tang inhibits IL-31/TRPV1/ERK1/2 signal axis and alleviates skin pruritus in uremic mice

作　　者：李灵睿 陈梦莹 游蔓芮 张家瑜 何竹[3] 王少清 沈宏春 LI Lingrui;CHEN Mengying;YOU Manrui(The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou Sichuan 646000,China;College of Integrated Traditional Chinese and Western Medicine,Southwest Medical University,Luzhou Sichuan 646000,China;Traditional Chinese Medicine Hospital of Leshan City,Leshan Sichuan 614000,China;Guang'an People's Hospital,Guang'an Sichuan 638500,China)

机构地区：[1]西南医科大学附属中医医院,四川泸州646000 [2]西南医科大学中西医结合学院,四川泸州646000 [3]乐山市中医医院,四川乐山614000 [4]广安市人民医院,四川广安638500 [5]成都医学院第二附属医院·核工业四一六医院,四川成都610000

出　　处：《四川中医》2024年第1期65-70,共6页Journal of Sichuan of Traditional Chinese Medicine

基　　金：泸州市科技计划项目(编号:2020LZXNYDJ27),成都市医学科研课题(编号:2021056)。

摘　　要：目的:阐明肤黄止痒汤对尿毒症皮肤瘙痒的疗效及其作用机制。方法:将40只小鼠随机分为5组(Sham、Model、FHZYT、BCTC、FHZYT+BCTC),每组8只,干预完成后计数小鼠搔抓次数。指标检测包括免疫组化染色检测皮肤中TNF-α、IL-1β、Substance P和IL-31的表达。WB检测皮肤中Substance P、TNF-α、IL-1β、IL-31、TRPV1、ERK1/2和p-ERK1/2的蛋白表达。结果:1.Model组小鼠肌酐、尿素氮水平升高,瘙痒次数增加(P<0.01)。2.Model组中TNF-α、IL-1β的表达上调,FHZYT、BCTC、FHZYT+BCTC组TNF-α表达则降低(P<0.01)。3.与Model组相比,FHZYT、BCTC、FHZYT+BCTC组瘙痒次数相对减少,皮肤中P物质、IL-31、TRPV1、ERK1/2及p-ERK1/2的表达水平降低(P<0.01)。结论:肤黄止痒汤可有效减少尿毒症小鼠皮肤瘙痒,其机制可能是通过抑制IL-31/TR⁃PV1/ERK1/2信号轴,改善皮肤炎症状态并发挥止痒作用。Objective:To elucidate the efficacy of Fu Huang Zhi Yang Tang on Uremic Pruritus and its mechanism of action.Methods:40mice were randomly divided into 5groups(Sham,Model,FHZYT,BCTC,FHZYT+BCTC),8mice in each group,and the number of scratching was counted after the intervention was completed.Indicator tests included immunohistochemical staining to detect the expression of TNF-α,IL-1β,Substance P and IL-31in the skin.WB to detect the protein expression of Substance P,TNF-α,IL-1β,IL-31,TRPV1,ERK1/2 and p-ERK1/2 in the skin.Results:1.Mice in the Model group had increased levels of creatinine and urea nitrogen,and the number of itching increased.2The expression of TNF-αand IL-1βwas up-regulated in the Model group,whereas the expression of TNF-αwas decreased in the FHZYT,BCTC,and FHZYT+BCTC groups.3Compared with the Model group,the number of itching in the FHZYT,BCTC,and FHZYT+BCTC groups was relatively The expression levels of substance P,IL-31,TRPV1,ERK1/2 and p-ERK1/2 in the skin were reduced.Conclusion:Fu Huang Zhi Yang Tang can effectively reduce itching in uremic mice,and its mechanism may be through the inhibition of IL-31/TRPV1/ERK1/2 signalling axis,which improves the inflammatory state of the skin and exerts the effect of itching relief.

关 键 词：尿毒症皮肤瘙痒 肤黄止痒汤 IL-31 TRPV1 ERK1/2 

分 类 号：R285.5[医药卫生—中药学]