RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs  

作　　者：Liyao Xiao Jiaxin Qiao Yiyang Huang Baohua Tan Linjun Hong Zicong Li Gengyuan Cai Zhenfang Wu Enqin Zheng Shanshan Wang Ting Gu 

机构地区：[1]National Engineering Research Center for Breeding Swine Industry,College of Animal Science,South China Agricultural University,Guangzhou 510000,China [2]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangzhou 510000,China [3]Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,Guangzhou 510000,China [4]Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding,Guangzhou 510000,China [5]Guangdong Wens Breeding Swine Technology Co.,Ltd.,Yunfu 527400,China [6]College of Life Science,Hubei University,Wuhan 430000,China

出　　处：《Acta Biochimica et Biophysica Sinica》2024年第3期452-461,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the 2023 Key Areas Research and Development Programs-Modern Seed Industry of Guangdong Province,China(No.2022B0202090002);the Provincial Rural Revitalization Strategy Special Project on the Protection and Development and Utilization of Local Livestock and Poultry of Guangdong Province,China.

摘　　要：Skeletal muscle is not only the largest organ in the body that is responsible for locomotion and exercise but also crucial for maintaining the body’s energy metabolism and endocrine secretion.The trimethylation of histone H3 lysine 27(H3K27me3)is one of the most important histone modifications that participates in muscle development regulation by repressing the transcription of genes.Previous studies indicate that the RASGRP1 gene is regulated by H3K27me3 in embryonic muscle development in pigs,but its function and regulatory role in myogenesis are still unclear.In this study,we verify the crucial role of H3K27me3 in RASGRP1 regulation.The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells(PSCs).The results of qPCR,western blot analysis,EdU staining,CCK-8 assay and immunofluorescence staining show that overexpression ofRASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models,while knockdown of RASGRP1 leads to the opposite results.These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mice and pigs.

关 键 词：RASGRP1 H3K27me3 cell proliferation MYOBLASTS skeletal muscle 

分 类 号：Q78[生物学—分子生物学]