蛭龙活血通瘀胶囊对糖尿病心肌病小鼠心功能的影响  

Effect of Zhilong Huoxue Tongyu Capsule on Cardiac Function of Diabetic Cardiomyopathy Mice

作　　者：杨方 刘平 陈皓灵 巫丹 董丽 YANG Fang;LIU Ping;CHEN Haoing;WU Dan;DONG Li(Department of Cardiology,the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou,Sichuan Province,646000 China)

机构地区：[1]西南医科大学附属中医医院心内科,四川泸州646000

出　　处：《糖尿病新世界》2024年第3期61-66,共6页Diabetes New World Magazine

基　　金：四川省科技厅重点研发计划(2022YFS0618)。

摘　　要：目的探讨蛭龙活血通瘀胶囊(Zhilong Huoxue Tongyu capsule,ZL)对糖尿病心肌病(Diabetic cardiomy-opathy,DCM)小鼠心功能的影响。方法本研究选取2023年2月1日开始于西南医科大学动物实验中心进行实验的27只C57BL/6J小鼠作为研究对象,按随机数表法分为对照组(n=9),实验组(n=18)。实验组小鼠采用链脲佐菌素(Streptozocin,STZ)结合高脂饮食喂养,建立DCM小鼠模型。建模成功后,实验组分为模型组,蛭龙活血通瘀胶囊组,每组8只。蛭龙活血通瘀胶囊组给予ZL 6.24 g/(kg·d)灌胃,其余组给予等体积生理盐水,连续6周。观察3组小鼠心脏质量指数、血脂、心肌组织病理、心脏彩超。结果给药6周后,蛭龙活血通瘀胶囊组小鼠心脏质量指数低于模型组,各项血脂指标及心功能指标优于模型组,差异有统计学意义(P均<0.05)。H&E染色结果显示,蛭龙活血通瘀胶囊组小鼠心肌间质胶原纤维数量较模型组显著减少。结论ZL具有抑制DCM小鼠心肌纤维化的作用,从而发挥心脏保护作用。Objective To investigate the effect of Zhilong Huoxue Tongyu capsule(ZL)on the cardiac function of dia-betic cardiomyopathy(DCM)mice.Methods This study selected 27 C57BL/6J mice from the Animal Experimental Center of Southwest Medical University on February 1,2023 as research objects,and were divided into control group(n=9)and experimental group(n=18)according to random number table method.The experimental group of mice are fed with Streptozocin(STZ)combined with a high-fat diet to establish a DCM model.After the successful modeling,the experimental group was divided into model group,Zhulong Huoxue Tongyu capsule group,8 animals in each group.The Zhilong Huoxuetongyu capsule group was given ZL 6.24 g/(kg·d)by gavage,and the other groups were given equal volume normal saline for 6 weeks.The heart mass index,blood lipid,cardiac tissue pathology and cardiac color ultrasound were observed in 3 groups.Results After 6 weeks of ZL treatment,the heart mass index of Zhilong Huoxue Tongyu capsule group was lower than that of the model group,and the blood lipid indicators and cardiac func-tion indicators were better than those of the model group,the differences were statistically significant(all P<0.05).H&E staining showed that the number of collagen fibers in the myocardial space of Bdellosaurus was significantly re-duced compared with the model group.Conclusion ZL can inhibit myocardial fibrosis in DCM mice and thus play a cardioprotective role.

关 键 词：蛭龙活血通瘀胶囊 糖尿病心肌病 心肌纤维化 心脏保护 

分 类 号：R587.1[医药卫生—内分泌]