白藜芦醇改善青春期酒精暴露导致的成年期大鼠认知功能损害  

Resveratrol Improves Cognitive Impairments Caused by Adolescent Intermittent Ethanol Exposure in Adult Rats

作　　者：贾文歌 刘媛 王心雨 李昊隆 曾令宇 杨一帆 李晨晨 陈红云 赵斌 魏来 JIA Wenge;LIU Yuan;WANG Xinyu;LI Haolong;ZENG Lingyu;YANG Yifan;LI Chenchen;CHEN Hongyun;ZHAO Bin;WEI Lai(Xinxiang Key Laboratory of Forensic Toxicology,College of Forensic Medicine,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院法医学院,新乡市法医毒理重点实验室,新乡453003

出　　处：《中国细胞生物学学报》2024年第3期436-444,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:U2004111);新乡医学院研究生科研创新支持计划(批准号:YJSCX202289Y);河南省高校大学生创新创业训练计划项目(批准号:202210472021)资助的课题。

摘　　要：该文旨在研究白藜芦醇(RSV)对青春期间歇性酒精(AIE)暴露大鼠认知功能的影响及潜在的机制。通过25%酒精灌胃建立AIE暴露大鼠模型,并使用RSV进行干预。旷场实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠进入中心区域的次数(P<0.01)、在中心区域活动距离减少(P<0.001)。RSV治疗后,Eth+RSV组大鼠较Eth+Veh组进入中心区域的次数(P<0.01)、在中心区域活动距离增加(P<0.05)。高架十字迷宫实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠开放臂停留时间(P<0.01)、进入开放臂次数显著减少(P<0.01)。RSV治疗后,Eth+RSV组大鼠较Eth+Veh组进入开放臂次数显著增加(P<0.05),在开放臂停留时间有增多趋势,但无统计学差异(P>0.05)。Morris水迷宫实验结果显示,与Con+Veh组相比,Eth+Veh组大鼠测试期逃避潜伏期增加(P<0.01)、穿越目标平台次数明显减少(P<0.05)。而RSV治疗后,Eth+RSV组大鼠较Eth+Veh组测试期逃避潜伏期降低(P<0.05)、穿越目标平台次数明显增多(P<0.05)。尼氏染色结果显示,与Con+Veh组相比,Eth+Veh组大鼠内侧前额叶皮层(mPFC)神经元数量显著减少(P<0.001)。与Eth+Veh组相比,Eth+RSV组大鼠mPFC神经元尼氏体数量显著增多(P<0.01)。Western blot结果显示,与Con+Veh组相比,Eth+Veh组大鼠mPFC内SIRT1(P<0.05)、BDNF(P<0.05)及PSD95(P<0.05)蛋白表达水平显著降低。与Eth+Veh组相比,Eth+RSV组大鼠mPFC内SIRT1(P<0.01)、BDNF(P<0.05)及PSD95(P<0.05)蛋白表达水平显著增高。该研究结果表明,RSV能够改善AIE导致的大鼠认知功能损害,并增加mPFC内神经元尼氏体数量,这种改善作用可能与mPFC中SIRT1、BDNF及PSD95的表达水平增加相关。This study aimed to investigate the effects of RSV(resveratrol)on cognitive function in rats exposed to AIE(adolescent intermittent ethanol)and its potential mechanisms.AIE-exposed rat models were es-tablished by intermittent gavage of 25%ethanol and the RSV or the Veh(vehicle)was administered intragastri-cally.The results of the open field test showed that compared with the Con+Veh group,the number of center zone entries(P<0.01)and the distance in center(P<0.001)of the Eth+Veh group were significantly reduced.After RSV treatment,compared with the Eth+Veh group,the number of center zone entries(P<0.01)and the distance in center(P<0.05)of the Eth+RSV group were significantly increased.The results of the elevated plus maze test showed that compared with the Con+Veh group,the open arm duration and the open arm entries of rats in the Eth+Veh group were significantly reduced(P<0.01).Compared with the Eth+Veh group,the open arm entries of rats in the Eth+RSV group were significantly increased(P<0.05),but the open arm entries were no statistical dif-ference(P>0.05).The results of the Morris water maze test showed that compared with the Con+Veh group,the latency to target of the Eth+Veh group was significantly increased(P<0.01)and the target crossings were signifi-cantly decreased(P<0.05).Compared with the Eth+Veh group,the latency to target and the target crossings of the Eth+RSV group were significantly decreased(P<0.05).The results of Nissl staining showed that compared with the Con+Veh group,the number of neurons in the mPFC(medial prefrontal cortex)in the Eth+Veh group was signifi-cantly decreased(P<0.001).Compared with the Eth+Veh group,the number of neurons in the Eth+RSV group was significantly increased(P<0.01).Western blot results showed that compared with the Con+Veh group,the protein expression levels of SIRT1(P<0.05),BDNF(P<0.05),and PSD95(P<0.05)in mPFC of rats in the Eth+Veh group were significantly decreased.Compared with the Eth+Veh group,the protein expression levels of SIRT1(P<0.01),BDNF(P<0.05),and PS

关 键 词：白藜芦醇 青春期间歇性酒精暴露 认知功能 沉默信息调节因子1 脑源性神经营养因子 突触后密度蛋白95 

分 类 号：R285.5[医药卫生—中药学]