小功率WPT电磁环境对海马CA1区神经元兴奋性的影响  

Effect of Low-Power WPT Electromagnetic Environment on Neuronal Excitability in Hippocampal CA1 Region

作　　者：赵军[1,2,3] 王晓轩 张冰茜 马菁 ZHAO Jun;WANG Xiaoxuan;ZHANG Bingqian;MA Jing(Hebei University of Technology Life Health and Engineering College,Tianjin 300130,China;Key Laboratory of Bioelectromagnetic and Neural Engineering of Hebei Province,Hebei University of Technology,Tianjin 300130,China;State Key Laboratory of Reliability and Intelligence of Electrical Equipment,Hebei University of Technology,Tianjin 300130,China)

机构地区：[1]河北工业大学生命科学与健康工程学院,天津300130 [2]河北省生物电磁与神经工程重点实验室(河北工业大学),天津300130 [3]天津市生物电工与智能健康重点实验室(河北工业大学),天津300130

出　　处：《中国细胞生物学学报》2024年第3期477-493,共17页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:51407058,52077057)的资助课题。

摘　　要：该研究旨在探究小功率无线电能传输(wireless power transmission,WPT)系统对小鼠海马CA1区神经元兴奋性的影响。将小鼠分为对照组和辐射组(2周组、4周组、6周组),通过莫里斯水迷宫实验、光纤光度实验、HE染色实验、膜片钳实验观察小鼠工作记忆能力、Ca^(2+)信号强度、海马锥体细胞数量、动作电位的变化以及瞬时外向K^(+)通道电流(I_(A))和延迟整流K^(+)通道电流(I_(K))的变化。莫里斯水迷宫实验结果显示,小功率WPT电磁环境不会对小鼠的工作记忆能力产生影响;光纤光度实验以及HE染色实验显示,小功率WPT电磁环境可能促进了海马CA1区神经元集群的放电活动,导致了荧光信号强度的增加。这表明电磁环境对Ca^(2+)浓度的调节可能增加了海马CA1区神经元放电活动次数,增强了海马CA1区神经元的兴奋性。随着辐射时间的增加,荧光信号的峰值逐渐下降,表明小鼠海马锥体细胞适应了小功率WPT电磁环境;小功率WPT电磁环境提高了小鼠海马CA1区的神经元的静息膜电位,缩短了动作电位的半波宽,降低了动作电位阈值,加快了海马CA1区的神经元动作电位的发放频率,促进了海马CA1区的神经元动作电位的发放,提高了海马CA1区的神经元的兴奋性;小功率WPT电磁环境会令细胞膜上的瞬时外向钾通道的激活过程受到抑制、延迟整流钾通道的激活特性向去极化方向移动,减少细胞内K^(+)的外流,进而增强海马CA1区神经元兴奋性。小功率WPT电磁环境可促进海马锥体细胞的放电活动,抑制I_(A)与I_(K)的激活过程,I_(A)通道的激活曲线向去极化方向偏移,从而增强神经元兴奋性。The aim of this study was to investigate the effects of low-power WPT(wireless power transmis-sion)system on the excitability of hippocampal CA1 neurons in mice.Mice were divided into control group and radiation group(2-week group,4-week group,6-week group).Morris water maze test,fiber photometry test,HE staining test,patch clamp test were used to observe the changes of working memory ability,Ca^(2+)signal,the number of hippocampal pyramidal cells,action potential,I_(A)(instantaneous outward K^(+)channel current)and I_(K)(delayed rectifier K^(+)channel current)in mice.The results of Morris water maze test showed that low-power WPT electro-magnetic environment did not affect the working memory ability of mice.Fiber photometry test and HE staining test showed that low-power WPT electromagnetic environment may promote the discharge activity of hippocampal CA1 neuron clusters,resulting in an increase in fluorescence signal.This suggests that the regulation of Ca^(2+)con-centration by electromagnetic environment might increase the number of discharge activities of hippocampal CA1 neurons and enhance the excitability of hippocampal CA1 neurons.With the increase of radiation time,the peak value of fluorescence signal gradually decreased,indicating that hippocampal pyramidal cells of mice adapted to low-power WPT electromagnetic environment.Low-power WPT electromagnetic environment increases the resting membrane potential of hippocampal CA1 neurons in mice,shortens the half-wave width of action potential,reduces the action potential threshold,accelerates the frequency of action potential release,promotes the release of action potential,and improves the excitability of hippocampal CA1 neurons.Low-power WPT electromagnetic environ-ment inhibits the activation process of transient outward potassium channels on the cell membrane,delays the acti-vation characteristics of rectifier potassium channels to the depolarization direction,reduces the outflow of K^(+)in the cell,and then enhances the excitability of hippocampal CA1 ne

关 键 词：海马锥体细胞 小功率WPT电磁环境 光纤光度实验 膜片钳技术 神经元兴奋性 

分 类 号：Q42[生物学—神经生物学]