Vaccinia virus viability under different environmental conditions and different disinfectants treatment  

作　　者：Shan Mei Liang Wei Yu Xie Fei Zhao Yu Huang Zhangling Fan Yamei Hu Liming Wang Lingwa Wang Ying Wang Fengwen Xu Fei Guo 

机构地区：[1]NHC Key Laboratory of Systems Biology of Pathogens,National Institute of Pathogen Biology and Center for AIDS Research,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100730,China [2]Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Bejing 100730,China [3]Clinical Laboratory,Emergency General Hospital,Beijing 100028,China

出　　处：《Biosafety and Health》2024年第1期21-27,共7页生物安全与健康（英文）

基　　金：supported by funds from the National Key R&D Program of China (2022YFE0203100);the CAMS Innovation Fund for Medical Sciences (CIFMS 2021-I2M-1-038 and CIFMS 2022-I2M-1-021);the National Natural Science Foundation of China (82271802,82241075,and 82072288).

摘　　要：Monkeypox(mpox)outbreak in 2022 has caused more than 91,000 cases,has spread to 115 countries,regions,and territories,and has thus attracted much attention.The stability of poxvirus particles in the environment is recognized as an important factor in determining their transmission.However,few studies have investigated the persistence of poxviruses on material surfaces under various environmental conditions,and their sensitivity to biocides.Here,we systematically measured the stability of vaccinia virus(VACV)under different environmental conditions and sensitivity to inactivation methods via plaque assay,quantitative real‐time polymerase chain reaction(qPCR),and Gaussia luciferase(G‐luciferase)reporter system.The results show that VACV is stable on the surface of stainless steel,glass,clothing,plastic,towel,A4 paper,and tissue and persists much longer at 4℃ and?20℃,but is effectively inactivated by ultraviolet(UV)irradiation,heat treatment,and chemical reagents.Our study raises the awareness of long persistence of poxviruses in the environment and provides a simple solution to inactivate poxviruses using common disinfectants,which is expected to help the control and prevention of mpox virus and future poxvirus outbreaks.

关 键 词：POXVIRUS Mpox virus Vaccinia virus PERSISTENCE DISINFECTANT 

分 类 号：R373[医药卫生—病原生物学]