2,5-二甲氧基-4-碘苯丙胺对自由活动小鼠次级视觉皮质V2区γ振荡的作用  

Effect of 2,5-dimethoxy-4-iodoamphetamine onγoscillations in area V2 of secondary visual cortex in freely moving mice

作　　者：王福旗 田京伟[1] 张树卓 苏瑞斌 WANG Fuqi;TIAN Jingwei;ZHANG Shuzhuo;SU Ruibin(School of Pharmacy,Yantai University,Yantai 264005,China;Beijing Key Laboratory of Neuro-psychopharmacology,State Key Laboratory of National Security Specially Needed Medicines,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]烟台大学药学院,山东烟台264005 [2]军事科学院军事医学研究院毒物药物研究所,国家安全特需药品全国重点实验室,神经精神药理学北京市重点实验室,北京100850

出　　处：《中国药理学与毒理学杂志》2024年第4期249-255,共7页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的研究5-羟色胺2A受体(5-HT2AR)激动剂2,5-二甲氧基-4-碘苯丙胺(DOI)对自由活动小鼠次级视觉皮质V2区场电位的影响。方法将C57BL/6J小鼠手术埋置皮质脑电(ECOG)电极,恢复7 d后ip给予1%DMSO(溶剂),记录次级视觉皮质V2区局部场电位(LFP)30 min;而后ip给予DOI 2.0 mg·kg^(-1),记录该区LFP 30 min;最后ip给予5-HT2AR拮抗剂氟利色林0.3 mg·kg^(-1),记录该区LFP 30 min。将数据导入Neuro Explorer软件进行频谱分析,用GraphPad Prism 8.0.2软件分析给药前后γ振荡和θ振荡功率谱密度变化,用Matlab软件分析θ振荡和γ振荡相位振幅耦合。结果与给予溶剂相比,给予DOI 2.0 mg·kg^(-1)可显著增高次级视觉皮质V2区γ振荡(30~80 Hz)功率(P<0.05)和θ振荡(4~7 Hz)功率(P<0.05),显著降低θ振荡和γ振荡相位振幅耦合调制指数(P<0.01);氟利色林可逆转DOI引起的次级视觉皮质V2区γ振荡和θ振荡功率的增高(P<0.05)。结论DOI的致幻作用可能与其增高小鼠次级视觉皮质V2区γ振荡和θ振荡功率及降低θ振荡和γ振荡相位振幅耦合有关。OBJECTIVE To investigate the effect of the 5-hydroxytryptamine 2A receptor(5-HT2AR)agonist 2,5-dimethoxy-4-iodoamphetamine(DOI)on field potentials in area V2 of the secondary visual cortex in freely moving mice.METHODS C57BL/6J mice were embedded with electroencephalocorti⁃cal(ECOG)electrodes and recovered for 7 d after surgery.After 1%DMSO(vehicle)was ip given and recording of local field potentials(LFP)in area V2 of the secondary visual cortex for 30 min,DOI(2.0 mg·kg^(-1),ip)was administered and LFP in area V2 was recorded for 30 min before the 5-HT2AR antagonist volinanserin(0.3 mg·kg^(-1),ip)was administered and LFP in area V2 was recorded for 30 min.The recorded data were imported into Neuro Explorer software for spectral analysis.GraphPad Prism 8.0.2 software was used to statistically analyze the changes in power spectral density ofγandθoscillations before and after administration while Matlab software was used to analyze the phase amplitude coupling ofθandγoscillations.RESULTS Compared with the vehicle group,DOI(2.0 mg·kg^(-1),ip)significantly increased the power ofγoscillations(30-80 Hz)andθoscillations(4-7 Hz)in area V2 of the secondary visual cortex(P<0.05),and the modulation index of phase amplitude coupling betweenθandγoscilla⁃tions was significantly reduced(P<0.01).The administration of volinanserin reversed the DOI-induced enhancement of the power ofγandθoscillations in area V2 of the secondary visual cortex(P<0.05).CONCLUSION DOI may exert its hallucinogenic effects by increasing the power ofγoscillations andθoscillations in area V2 of the secondary visual cortex and by decreasing the phase amplitude coupling ofθoscillations toγoscillations in mice.

关 键 词：次级视觉皮质 γ振荡 皮质脑电 2 5-二甲氧基-4-碘苯丙胺 局部场电位 

分 类 号：R964[医药卫生—药理学]