聚酪氨酸/纳米金电化学传感器检测水环境中环丙沙星  

Detection of Ciprofloxacin in water based on polytyrosine/nano gold electrochemical sensor

作　　者：秦建芳[1] 李威威 张玉莹 白乙艳 王龙 武舒敏 杨海英[1] QIN Jian-fang;LI Wei-wei;ZHANG Yu-ying;BAI yi-yan;WANG Long;WU Shu-min;YANG Hai-ying(Department of Chemistry,Yuncheng University,Yuncheng 044000,China;Shanxi Qiyu Environmental Protection New Material Research Institute Co.LTD,Yuncheng 044000,China)

机构地区：[1]运城学院应用化学系,山西运城044000 [2]山西奇宇环保新材料研究院有限公司,山西运城044000

出　　处：《化学研究与应用》2024年第4期751-759,共9页Chemical Research and Application

基　　金：山西省省筹留学人员回国科研项目(2020-140)资助;山西省应用基础研究自由探索类青年项目(202203021212173)资助;山西省教育厅高校科技创新类项目(2022L475)资助;运城市科技局应用研究项目(YCKJ-2022065,YCKJ-2021027)资助;优秀博士来晋科研项目(QZX-2023008)资助。

摘　　要：利用聚酪氨酸有机薄膜负载金纳米粒子作为检测环丙沙星的基底材料,制备了p-Tyr/AuNP s/Au电化学传感器,对环丙沙星(CFX)表现出良好的电催化性能。用扫描电镜对材料的形貌进行了表征,CFX在该材料表面的反应为二电子转移的受吸附控制的过程。用单因素实验对L-酪氨酸(L-Tyr)单体的浓度、聚合的扫描圈数、金纳米沉积时间、溶液pH值进行了优化。在最优条件下,在6.60×10^(-7)~1.57×10^(-5)mol·L^(-1),ΔI与环丙沙星的浓度呈良好的线性关系,检出限为3.50×10^(-7)mol·L^(-1)(S/N=3)。该电化学传感器具有良好的重复性、稳定性和抗干扰性,可用于实际水体中环丙沙星的测定,加标回收率为95.7%~103.5%。Using polytyrosine organic film loaded with gold nanoparticles as the substrate for ciprofloxacin detection,the electrochemical sensor p-Tyr/AuNPs/Au was constructed.It showed good electrocatalytic performance for Ciprofloxacin(CFX).The surface morphology of the composite film was characterized by scanning electron microscope.The reaction of CFX on the surface is an adsorption-controlled process of two electron transfer.The experimental conditions such as the concentration of L-tyrosine solution,the number of scanning cycles of polytyrosine,the deposition time of gold nanoparticles and the pH value of the solution were optimized by single factor experiment.Under the optimal conditions,good linear relationship was obtained for CFX at range of 6.60×10^(-7)to 1.57×10^(-5)mol·L^(-1),with the detection limit(S/N=3)of 0.35μmol·Ll.Accordingly,the reproducibility,stability and an-ti-interference tests were carried out.Furthermore,the designed sensor was successfully applied to detect CFX in actual water samples and the recovery was found to be 95.7%~103.5%.

关 键 词：p-Tyr/AuNPs/Au 金纳米 聚酪氨酸 环丙沙星 

分 类 号：O657.1[理学—分析化学]