载阿霉素光响应纳米锆基MOFs构建及细胞毒性研究  

Construction and cytotoxicity of doxorubicin-loaded photoresponsive nano-zirconium-based MOFs

作　　者：艾璞 占思研 李晖[1] 孙巧媚 AI Pu;ZHAN Si-yan;LI Hui;SUN Qiao-mei(College of Chemical Engineering,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学化工学院,四川成都610065

出　　处：《化学研究与应用》2024年第4期851-858,共8页Chemical Research and Application

基　　金：国家自然科学基金项目(22108180)资助;中国博士后科学基金项目(2022M712232)资助。

摘　　要：为了克服乳腺癌治疗药物阿霉素存在毒副作用大、作用时间短等限制,本研究采用含偶氮苯侧链结构的联苯羧酸与Zr4+构建了一种新型光响应纳米金属有机框架(U67-AZO-COOH)作为药物载体,成功地将阿霉素负载其中。通过粉末X射线衍射、扫描电镜、傅里叶变换红外光谱、全自动比表面及孔隙度分析等技术手段对其结构进行了详细表征,得到在紫外光照射下阿霉素的载药率可由18.57%降至7.31%。体外释放实验和细胞毒性实验结果表明,该纳米药物复合物具有高载药量、良好的生物相容性,其在微酸环境下的释放率57.02%高于生理条件39.26%,表现出显著的缓释效果,能够减少对正常细胞的损害,存活率从39.88%提高到81.73%。这为新型光响应纳米金属有机框架在药物传递领域的广泛应用提供了新的可能性。To address the significant toxic side effects and short duration of action associated with the breast cancer treatment drug doxorubicin,this study utilized a novel photo-responsive nano-metal-organic framework(U67-AZO-COOH).Constructed with azobenzene-containing benzoic acid and Zra as a drug carrier,the framework successfully encapsulated doxorubicin.The structure underwent comprehensive characterization using techniques such as powder X-ray difraction,scanning electron microscopy,Fouriertransform infrared spectroscopy,and automated surface area and pore size analysis.Under ultraviolet light exposure,the doxorubicin loading rate decreased from 18.57%to 7.31%.In vitro release experiments and cytotoxicity assays revealed that this nano-drug composite exhibited a high drug loading capacity and excellent biocompatibility.The release rate in a mildly acidic environment(57.02%)surpassed the physiological condition rate(39.26%),demonstrating a significant sustained release effect.This capability effectively reduced damage to normal cells,with the survival rate increasing from 39.88%to 81.73%.These findings offer new avenues for the widespread application of novel photo-responsive nano-metal-organic frameworks in the realm of drug delivery,meeting the requirements of English-language journals.

关 键 词：金属有机框架 光响应 纳米载药材料 阿霉素 乳腺癌 

分 类 号：O614.4[理学—无机化学]