一种基于表面增强拉曼光谱技术的环境水中啶虫脒现场快速检测试剂盒  

A portable kit for rapid on-site detection of acetamiprid in environmental water based on surface-enhanced Raman spectroscopy

作　　者：王硕 邹薇[1] 张梦萍[1] 张鑫垚 王辉[1] 于颐光 王瑞[1] 王翠娟[1] 张宏[1] WANG Shuo;ZOU Wei;ZHANG Meng-ping;ZHANG Xin-yao;WANG Hui;YU Yi-guang;WANG Rui;WANG Cui-juan;ZHANG Hong(Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250062,China)

机构地区：[1]山东省职业卫生与职业病防治研究院,山东第一医科大学(山东省医学科学院),山东济南250062

出　　处：《化学研究与应用》2024年第4期898-904,共7页Chemical Research and Application

基　　金：国家自然科学基金项目(81872575)资助;山东省自然科学基金项目(ZR202211280423,2017YL002)资助;山东省中医药科技项目(2021M152,M-2022226)资助;山东省医药卫生科技发展计划项目(202112070425,202115020461,202212010448)资助;济南市临床医学科技创新计划项目(202225062)资助。

摘　　要：啶虫脒(acetamiprid,AC)在环境中的蓄积可对生态系统和人体健康产生不利影响。本研究利用表面增强拉曼光谱(Surface-enhanced Raman spectroscopy,SERS)技术,构建了一种快速定性及定量检测环境水中啶虫脒的检测试剂盒。采用化学合成法制备了粒径为40~60 nm的纳米金溶胶作为SERS活性基底,以包覆铝箔的24孔板为反应平台,将一定量的聚沉盐负载到反应孔中诱导信号热点,并与便携式拉曼光谱仪集成为适用于现场检测的试剂盒。通过对聚沉盐负载量、聚沉时间等条件进行优化,在浓度为25~750 ng·mL^(-1)范围内,啶虫脒浓度与SERS信号强度呈现良好的线性关系,R2=0.988。试剂盒检出限低至15.56 ng·mL^(-1),平均加标回收率为90.04%~106.10%,在5 min内实现了环境水中啶虫脒的现场检测。与传统方法相比,该试剂盒便携、操作简单、快速、成本低廉、可同时进行多样本检测,为环境水体中啶虫脒的现场检测分析提供了新选择。The accumulation of acetamiprid(AC)in the environment can adversely affect ecosystems and human health.This study constructed a portable kit for the rapid qualitative and quantitative detection of AC in environmental water.Gold colloid with a 40-60 nm particle size was prepared using the chemical synthesis method and used as the SERS substrate.An aluminum foil was placed on a 24-hole plate to serve as a bearing platform,Additionally,a certain amount of aggregation salt was loaded to induce signal hot spots.All the SERS signals were collected using a portable Raman spectrometer.In this study,aggregating conditions,such as the type and concentration of salts,were optimized.A linear relationship between AC concentration and SERS's signal strength was observed,with an R?value of 0.988.The detection limit of this kit was calculated to be 15.56 ng·mL^(-1).The average recovery rate ranged from 90.04%to 106.10%.Compared with the traditional method,the kit is portable,simple to operate,rapid,and inexpen-sive.It can detect multiple samples simultaneously,providing a new alternative for the field detection of AC in environmental water.

关 键 词：表面增强拉曼光谱 便携式试剂盒 快速检测 啶虫脒 纳米金胶体 

分 类 号：O657.3[理学—分析化学]