刺糖低聚糖的提取分离与结构鉴定  

Extraction,Isolation and Structural Identification of An Oligosaccharide from Alhagi Honey

作　　者：宋建忠 吕志远 杨婧[3] 陈盈盈 赵欣 陈章浩 李改茹[1] 常军民[1,5] SONG Jian-zhong;LYU Zhi-yuan;YANG Jing;CHEN Ying-ying;ZHAO Xin;CHEN Zhang-hao;LI Gai-ru;CHANG Jun-min(College of pharmacy,Xinjiang Medical University,Urumqi 830000,China;Department of Pharmacy,Tumor Hospital Affiliated to Xinjiang Medical University,Urumqi 830011,China;The 63650th Hospical of PLA,Urumqi 830000,China;Department of Pharmacy,the First Afffliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Xinjiang Key Laboratory of Natural Drug Active Components and Drug Release Technology,Urumqi 830000,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830000 [2]新疆医科大学附属肿瘤医院药学部,新疆乌鲁木齐830011 [3]中国人民解放军63650部队医院,新疆乌鲁木齐830000 [4]新疆医科大学第一附属医院药学部,新疆乌鲁木齐830054 [5]新疆天然药物活性组分与释药技术重点实验室,新疆乌鲁木齐830000

出　　处：《食品与药品》2024年第2期148-152,共5页Food and Drug

基　　金：新疆维吾尔自治区重大科技专项(编号:2022A03019-3)。

摘　　要：目的从刺糖中分离纯化获得刺糖低聚糖单体,并对其进行结构鉴定。方法采用多种色谱方法对刺糖提取物进行分离、纯化,运用高效液相色谱-电喷雾离子阱质谱(HPLC/ESI-MS)、红外光谱(IR)及核磁共振波谱(NMR)等技术,对所得刺糖低聚糖单体进行结构鉴定。结果经分离纯化得到1个刺糖低聚糖单体,相对分子质量为504,结构为α-D-葡萄糖-(1→6)-β-D-葡萄糖-(1→6)-D-葡萄糖,经查证定性为低聚异麦芽糖。结论本研究首次从刺糖中分离纯化出低聚异麦芽糖。Objective To isolate and purify an oligosaccharide monomer from Alhagi honey and elucidate its molecular structure.Methods Various chromatographic techniques were employed to extract and purify Alhagi honey extract.The molecular structure of the isolated Alhagi honey oligosaccharide was determined using high-performance liquid chromatography-electrospray ionization mass spectrometry(HPLC/ESI-MS),infrared spectroscopy(IR),and nuclear magnetic resonance spectroscopy(NMR).Results After separation and purification,one oligosaccharide monomer with relative molecular weight of 504 and structure ofα-D-Glc-(1→6)-β-D-Glc-(1-6)-D-Glc was obtained,which was confirmed to be isomaltooligosaccharide.Conclusion In this study,isomaltooligosaccharide is isolated and purified from Alhagi honey for the first time.

关 键 词：刺糖 低聚糖 核磁共振波谱 结构解析 

分 类 号：R284.1[医药卫生—中药学]