酸处理·酶制剂对杏鲍菇菌渣还原糖和纤维素降解的影响  

Effects of Acid Treatment and Enzymatic Hydrolysis on Reducing Sugar and Cellulose Degradation of Pleurotus eryngii Residue

作　　者：王岩 于洪敏 李娟 刘艳 江国托 WANG Yan;YU Hong-min;LI Juan(Dalian Sanyi Animal Medicine Co.,Ltd.,Dalian,Liaoning 116036)

机构地区：[1]大连三仪动物药品有限公司,辽宁大连116036

出　　处：《安徽农业科学》2024年第8期86-91,共6页Journal of Anhui Agricultural Sciences

基　　金：2019年大连市青年科技之星项目(2019RQ146)。

摘　　要：为探究酸处理(酸浓度、时间、温度)、酶制剂(纤维素酶、木聚糖酶、β-葡聚糖酶)对杏鲍菇菌渣的影响,以河北采集的杏鲍菇菌渣为原料,以总还原糖含量为研究指标,通过主成分分析方法研究影响杏鲍菇菌渣酸处理的因素;以中性洗涤纤维减少量和酸性洗涤纤维减少量为响应值,开展正交试验,分析酶制剂对其的影响。结果表明:40℃条件下,2%和3%酸浓度处理随时间的延长会降解总还原糖;60℃条件下,酸浓度的增加可以减缓总还原糖的降解;80℃条件下,1%和2%酸浓度处理可以促进多糖的降解,提高总还原糖含量。根据主成分分析结果可知,温度与总还原糖含量呈正相关,是影响杏鲍菇菌渣多糖降解的最主要因素。正交试验结果表明,木聚糖酶添加量和纤维素酶添加量是影响中性洗涤纤维和酸性洗涤纤维降解的最主要因素,最优酶解配方为纤维素酶85 U/mL、木聚糖酶220 U/mL、葡聚糖酶40 U/mL,说明菌渣经酸、酶处理后可作为反刍饲料。In order to explore the effects of acid treatment(acid concentration,time and temperature),enzyme preparations(cellulase,xylanase,β-glucanase)on Pleurotus eryngii fungus residue.The experiment used the mushroom residue of Pleurotus eryngii collected in Hebei Province as the raw material,total reducing sugar content as the research index,the factors affecting the acid treatment of P.eryngii residue were analyzed by principal component analysis.At the same time,the effects of enzyme preparation on the reduction of neutral detergent cellulose and acid detergent cellulose were analyzed by orthogonal test.At 40°C,2%and 3%acid concentrations would degrade the content of reducing sugar with time.At 60°C,the increase of acid concentration could slow down the degradation of total reducing sugar.At 80℃,1%and 2%acid concentrations could promote the degradation of polysaccharides and increase the content of total reducing sugar.According to the principal component analysis,temperature was the most important factor affecting the content of reducing sugar of P.eryngii slag.The orthogonal test results showed that the degradation of xylanase and cellulase were the main factors affecting the reduced amount of neutral detergent cellulose and acid detergent cellulose respectively.The optimal enzymolysis formula was cellulase 85 U/mL,xylanase 220 U/mL and glucanase 40 U/mL.These results indicated that the bacterial residue after acid and enzyme treatment has nutritional value as a ruminant feed.

关 键 词：杏鲍菇菌渣 酸处理 酶制剂 还原糖 纤维素 

分 类 号：S548[农业科学—作物学]