Determination of Chlorogenic Acid,Geniposide,Total Flavonoids and Total Triterpenes in Wulan-13  

作　　者：Xiaohua WU Burenbatu 

机构地区：[1]College of Clinical Medicine,Inner Mongolia Minzu University,Tongliao 028000,China [2]Affiliated Hospital of Inner Mongolia Minzu University,Tongliao 028000,China

出　　处：《Medicinal Plant》2024年第2期6-9,共4页药用植物：英文版

基　　金：Supported by National Natural Science Foundation of China(82274210).

摘　　要：[Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposide were determined by HPLC,and the contents of total flavonoids and total triterpenes were determined by an ultraviolet spectrophotometer.[Results]There was a good linear relation between the mass of chlorogenic acid reference substance and the peak area in the range of 0.05-0.45μg,and the regression equation was Y=2524.1X+3.1943,(r=0.9998).A good linear relationship was found between the mass of gardenoside reference substance and the peak area in the range of 0.776-6.984μg,and the regression equation was Y=1670.5X+64.804,(r=0.9998).There was also a good linear relation between the mass of rutin reference substance and its absorbance in the range of 0.00808-0.04848 mg,and the regression equation was Y=12.916X+0.014,(r=0.999).The mass of oleanolic acid reference substance had a good linear relation with its absorbance in the range of 0.00418-0.0209 mg,and the regression equation was Y=51.89X-0.0839,(r=0.9991).[Conclusions]The content determination method is simple,reliable and reproducible,and suitable for controlling the contents of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.

关 键 词：Wulan-13 HPLC Ultraviolet spectrophotometer Content determination 

分 类 号：R284.2[医药卫生—中药学]