TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone  

作　　者：Jiangcun WEI Xiumei MA Meiyan QIU Bing QING Jingrong LU Hong LEI Xiaodong HUANG Wen ZHONG 

机构地区：[1]Guangxi International Zhuang Medical Hospital,Nanning 530201,China [2]Guangxi University of Chinese Medicine,Nanning 530200,China

出　　处：《Medicinal Plant》2024年第2期15-17,20,共4页药用植物：英文版

基　　金：Supported by Self-funded Research Project of Administration of Traditional Chinese Medicine of Guangxi Zhuang Autonomous Region(GXZYA20220171);Young and Middle-aged Teachers Research Basic Ability Improvement Project of Colleges and Universities in Guangxi(2022KY0307);General Project of Guangxi University of Chinese Medicine(2022MS038);"Qingmiao Project"Talent Cultivation Program of Guangxi International Zhuang Medical Hospital(2022001);Key Project of Guangxi International Zhuang Medical Hospital(GZ2021010);High-level TCM Key Discipline(Zhuang Medical Science)Construction Project of State Administration of Traditional Chinese Medicine(zyyzdxk-2023165);Key Research and Development Project of Guangxi Provincial Department of Science and Technology(GK AB21196057);High-level Talent Cultivation Innovation Team Funding Project of Guangxi University of Chinese Medicine(2022A008).

摘　　要：[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.

关 键 词：Pileostegia tomentellal TLC identification Extraction technology UMBELLIFERONE Content determination 

分 类 号：R284[医药卫生—中药学]