The Mechanism by Which Pereskia aculeata Mill.Regulates Ferroptosis and Interferes with Colon Cancer Based on Network Pharmacology  

作　　者：Kaifei LIU Yingyuan QIN Linhao LIU Ye CHU Huanjin HUANG Jingwei LUO Xiaolong CHEN Yang YANG Yifei CHEN 

机构地区：[1]College of Pharmacy,Guilin Medical University,Guilin 541199,China [2]Guangxi Key Laboratory of Drug Discovery and Optimization,Guangxi Engineering Research Center for Pharmaceutical Molecular Screening and Druggability Evaluation,College of Pharmacy,Guilin Medical University,Guilin 541199,China [3]Department of Nephrology,Guilin Municipal Hospital of Traditional Chinese Medicine,Guilin 541002,China

出　　处：《Medicinal Plant》2024年第2期26-29,共4页药用植物：英文版

基　　金：Supported by National Natural Science Foundation of China(82160832);the Open Project Program of Guangxi Key Laboratory of Brain and Cognitive Neuroscience,Guilin Medical University(GKLBCN-202206-02);Annual Scientific Research Project of Guangdong Provincial Administration of Traditional Chinese Medicine in 2022(20222138);the Fourth Training Plan for Thousands of Young and Mid-aged Mainstay Teachers in Guangxi Colleges and Universities;the Innovation and Entrepreneurship Training Program for College Students in Guilin Medical University in 2023(X202310601232,S202310601140,202210601214).

摘　　要：[Objectives]The paper was to investigate the mechanism by which Pereskia aculeata Mill.regulates ferroptosis and interferes with colon cancer using network pharmacology.[Methods]The effect of P.aculeata on HCT116 cells was observed by examining cell morphology.The intersection of P.aculeata,colon cancer,and ferroptosis targets was determined using the Venny 2.1.0 online platform.DAVID database was used to perform GO and KEGG enrichment analyses.Cytoscape_v3.10.0 was used for network mapping and PPI analysis.[Results]The observation of cell morphology indicated that P.aculeata suppressed the growth of HCT116 cells.GO and KEGG analysis identified 67 genes involved in potential pathways,including the MAPK signaling pathway and Apoptosis,18 of which were located on the MAPK pathway.After conducting PPI visualization analysis and ranking based on Degree values,we identified TP53,TNF,EGFR,MAPK14,and AKT1 as the top five targets with the highest Degree values.[Conclusions]P.aculeata may activate the p53 gene through the MAPK signaling pathway,inducing ferroptosis and ultimately resulting in tumor cell death.

关 键 词：Pereskia aculeata Mill. Ferroptosis MAPK signal pathway Colon cancer 

分 类 号：R285[医药卫生—中药学]