2018年~2021年河北省部分地区鸡源沙门菌的生物学特性及致病性研究  

Epidemiological investigation of chicken-derived Salmonella in parts of Hebei Province from 2018 to 2021

作　　者：李涵 赵允清 谢相悦 庄洪霞 王璐 苏蒙蒙 韩平 成文武 刘永波 张艳英[1] LI Han;ZHAO Yun-qing;XIE Xiang-yue;ZHUANG Hong-xia;WANG Lu;SU Meng-meng;HAN Ping;CHENG Wen-wu;LIU Yong-bo;ZHANG Yan-ying(Hebei Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Hebei Broiler Breeding and Processing Technology Innovation Center,Hebei Luanping Huadu Food Co.,Ltd.,Chengde 067000,China)

机构地区：[1]河北科技师范学院、河北省预防兽医学重点实验室,河北秦皇岛066004 [2]河北省肉鸡养殖与加工技术创新中心、河北滦平华都食品有限公司,河北承德067000

出　　处：《中国预防兽医学报》2024年第1期16-23,31,共9页Chinese Journal of Preventive Veterinary Medicine

基　　金：河北省高等教育科学技术项目(ZD20190920);河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(HBCT2013090203);博士研究启动基金项目(2022YB006);河北科技师范学院动物科技学院基金项目(DK2020-4);河北省肉鸡养殖与加工技术创新中心科研项目。

摘　　要：为调查河北地区鸡源沙门菌及其血清型的流行及分布情况,并分析其的致病性,本研究于2018年~2021年在河北省7个地区34个养鸡场共收集631份发病鸡肛拭子和62份病料样品共计693份,分别接种普通琼脂培养基分离细菌并纯化后经革兰氏染色镜检;将分离菌分别接种不同的培养基培养,并对分离菌经生化及16S r RNA基因的PCR及测序鉴定,随机选择32条测序序列经NCBI的BLAST比对,并采用DNAStar软件分析该基因的同源性。采用玻片凝集法鉴定分离菌的血清群及血清型并统计各血清群与血清型在河北不同地区的分布。结果显示,从693份病料样品中共分离到238株沙门菌,除3株未定群外,其余分离菌分属于A、B、C、D、E、F 6个血清群,其中D群最多达69.7%(166/238);238株沙门菌分属于7个不同的血清型和未定型,依次为鸡白痢沙门菌(91/38.2%)、甲型副伤寒沙门菌(43/18.0%)、鸡伤寒沙门菌(40/16.8%)、肠炎沙门菌(39/16.4%)等。血清群与血清型分布的统计结果显示,除张家口地区流行A群血清群外,其余地区流行的血清群均为D群;张家口地区流行鸡白痢沙门菌;秦皇岛地区有14种血清型,其中甲型副伤寒沙门菌为其流行血清型;石家庄、承德、唐山流行的均为鸡白痢沙门菌,邢台及保定地区分别流行肠炎沙门菌与鸡伤寒沙门菌。通过K-B药敏纸片法检测238株分离菌对7类共22种药物的敏感性;采用PCR检测分离菌的相关耐药基因并采用SPSS26.0软件中的Fisher确切概率法分析分离菌耐药表型与耐药基因之间的相关性。选取12株不同血清型的分离菌以0.2 m L/只(3×108cfu/mL)感染雏鸡,分析各分离菌对雏鸡的致病性。药敏试验结果显示,对苯唑西林、红霉素、青霉素、甲氧苄啶、氨苄西林、四环素耐药的菌株分别占97.5(232/238)、89.1%(212/238)、78.6%(187/238)、74.8%(178/238)、74.4%(177/238)、64.3%(153/238),对其它药物耐药的菌株均在32%�To investigate the prevalence and distribution of Salmonella and its serotypes in chickens in Hebei Province,and to analyze its pathogenicity,a total of 631 anal swabs and 62 diseased tissues were collected from 34 chicken farms in 7 regions of Hebei Province from 2018-2021,totaling 693 samples.The samples were inoculated into ordinary agar medium for isolation and purification of the pathogenic bacteria,followed by Gram staining and microscopy.The isolates were inoculated in different medium respectively,and the isolates were biochemically identified.PCR and sequencing of 16S rRNA gene were used to identify the isolates,and 32 sequencing sequences were randomly selected for sequence alignment by BLAST of NCBI,and the homology of the gene was analyzed by DNAStar software.The serogroups and serotypes of the isolates were identified by slide agglutination method,and the distribution of each serogroup and serotype in different regions of Hebei Province was counted.The results showed that 238 strains of Salmonella were isolated from 693 samples of diseased chickens.Except for 3 strains of undetermined group,the rest of the 238 isolates belonged to 6 serogroups A,B,C,D,E,and F,of which group D was at most 69.7%(166/238).The 238 strains of Salmonella were classed into 7 different serotypes and 1 undefined type,with the top three being Salmonella Pullorum(91/38.2%),Salmonella Paratyphi A(43/18.0%),Salmonella Gallinarum(40/16.8%).The statistical results of the distribution of serogroups and serotypes of isolates showed that except for the serogroup A circulating in Zhangjiakou area,the serogroups prevalent in other regions were all serogroup D.Salmonella Pullorum was the dominant serotype in Zhangjiakou area;There were 14 serotypes in Qinhuangdao,among which Salmonella Paratyphi A was its dominant serotype.The prevalent serotypes in Shijiazhuang,Chengde and Tangshan were all Salmonella Pullorum,and the serotypes dominant in Xingtai and Baoding were Salmonella Enteritidis and Salmonella Typhimurium,respectively.The suscep

关 键 词：鸡源沙门菌 血清型鉴定 药物敏感性试验 耐药基因 耐药性分析 

分 类 号：S852.61[农业科学—基础兽医学]