华西广医1号急性毒性、鼻黏膜刺激性和增强免疫功能的研究  

Acute Toxicity,Nasal Mucosa Stimulation and Enhancement of Immune Function of Huaxi Guangyi No.1

作　　者：杨宁[1] 李轶 彭成[2] 张明 谢晓芳[2] YANG Ning;LI Yi;PENG Cheng;ZHANG Ming;XIE Xiao-fang(Guang an People s Hospital,Guang an 638001,China;Chengdu University of Traditional Chinese Medicine/State Key Laboratory of Traditional Chinese Medicine Resources in Southwest China,Chengdu 611137,China;Department of Pharmacy,Nanchong Traditional Chinese Medicine Hospital,Nanchong 637000,China)

机构地区：[1]广安市人民医院,广安638001 [2]成都中医药大学/西南特色中药资源国家重点实验室,成都611137 [3]南充市中医医院制剂中心,南充637000

出　　处：《科学技术与工程》2024年第10期4020-4027,共8页Science Technology and Engineering

基　　金：四川省中医药管理局项目(2020CP0011);国家中医药多学科交叉创新团队项目(ZYYCXTD-D-202209);四川省中医药科技产业创新团队项目(2022C001)。

摘　　要：为初步了解拟开发医院雾化制剂华西广医1号的安全性和有效性,通过小鼠急性毒性试验、大鼠鼻黏膜刺激性试验和增强免疫功能的药效学试验方法研究了华西广医1号安全性和药效。结果表明:以0.34 g/mL药液24 h内雾化给药,对小鼠无明显急性毒性;对大鼠经鼻腔给药无明显刺激性;以0.068 g/mL雾化给药,有升高幼龄小鼠的胸腺指数的作用趋势;可升高环磷酰胺致免疫功能低下小鼠的巨噬细胞碳粒廓清指数K、校正指数α和外周血白细胞数目(white blood cell,WBC),红细胞数目(red blood cell,RBC)和血红蛋白浓度(hemoglobin,HGB)水平,并可升高溶血素值。可见,华西广医1号雾化给药小鼠的最大给药量为34 g/kg,无明显鼻黏膜刺激性,0.068 g/mL雾化给药有增强免疫功能,可用于预防上呼吸道感染。In order to evaluate the safety and efficacy of the hospital atomization preparation Huaxi Guangyi No.1 to be developed,the safety of Guangyi No.1 was used to investigate by acute toxicity test in mice and nasal mucosa irritation test in rat.The efficacy of Guangyi No.1 was used to investigate by pharmacodynamic test for enhancing immune function.The results show that there is no obvious acute toxicity to mice when 0.34 g/mL of the drug solution was given by atomization within 24 hours.There was no obvious irritation of intranasal administration in rats.Aerosolized administration at 0.068 g/mL tended to increase the thymic index of young mice.It can increase the carbon clearance index K and correction indexαof macrophages and the levels of WBC and HGB in peripheral blood of immunocompromised mice induced by cyclophosphamide,and increase the value of hemolysin.It is concluded that the maximum dose of Huaxi Guangyi No.1 aerosol administration in mice is 34 g/kg,and there is no obvious irritation of nasal mucosa.The 0.068 g/mL aerosol administration can enhance immune function and can be used to prevent upper respiratory tract infection.

关 键 词：华西广医1号 玉屏风散 雾化 上呼吸道感染 急性毒性 刺激性 免疫功能 

分 类 号：R965.3[医药卫生—药理学]