新型硫酸盐还原菌的分离鉴定及其对六价铬污染物的原位修复效果  

ISOLATION AND IDENTIFICATION OF A NEW SULFATE-REDUCING BACTERIUM AND ITS IN SITU REMEDIATION EFFECT OF HEXAVALENT CHROMIUM-CONTAMINATED SOIL

作　　者：韩建均 柴陆军 王国金[3] 张瑜 秦康佳 周曼 梁雪杰 郝俊鹏 王慧[4] HAN Jianjun;CHAI Lujun;WANG Guojin;ZHANG Yu;QIN Kangjia;ZHOU Man;LIANG Xuejie;HAO Junpeng;WANG Hui(Research Institute for Environmental Innovation(Suzhou)Tsinghua,Suzhou 215010,China;Beijing Bennong Technology Development Co.,Ltd,Beijing 100176,China;Shangdong Branch of China National Geological Exploration Center of Building Materials Industry,Jinan 250100,China;Tsinghua University,Beijing 100091,China)

机构地区：[1]清华苏州环境创新研究院,江苏苏州215010 [2]北京本农科技发展有限公司,北京100176 [3]中国建筑材料工业地质勘查中心山东总队,济南250100 [4]清华大学,北京100091

出　　处：《环境工程》2024年第2期192-198,共7页Environmental Engineering

基　　金：国家重点研发计划“污染场地大数据管理分析研究及平台构建”(2020YFC1807402)。

摘　　要：利用微生物将六价铬[Cr(Ⅵ)]还原为三价铬[Cr(Ⅲ)]是一种具有前景的铬污染土壤修复方法。从新型的分离和分子生物学鉴定着手,通过含铬溶液和铬污染土壤试验探讨了菌株对Cr(Ⅵ)的修复效果。结果表明:分离得到能够还原Cr(Ⅵ)的硫酸盐还原菌S-7,其16S rDNA全长序列与Desulfovibrio desulfuricans DSM 642T存在98.89%相似度,属于新型脱硫弧菌种,命名为Desulfovibrio desulfuricans S-7。D.desulfuricans S-7生长繁殖的最适合生长温度为30℃,最适合pH值是7.0,耐NaCl浓度范围为0~10%。D.desulfuricans S-7修复铬溶液的结果表明:对25 mg/L的Cr(Ⅵ)去除率为79.74%,在100 mg/L的Cr(Ⅵ)条件下D.desulfuricans S-7的生长繁殖受到抑制。Cr(Ⅵ)污染土壤实验的结果表明:土壤中Cr(Ⅵ)含量50.0 mg/kg降到3.1 mg/kg,去除率达到93.8%,修复后的土地质量符合GB 36600—2018一类建设用地的标准。研究表明:D.desulfuricans S-7能够高效地原位修复厌氧环境下Cr(Ⅵ)污染土壤,为原位修复深层Cr(Ⅵ)污染土壤提供一种新的替代方案。Microbial reduction of Cr(Ⅵ)to Cr(Ⅲ)is a promising method for remediation of chromium-contaminated soil.Molecular biology was used to identify the species of the strain,and the in situ remediation effect of hexavalent chromium was evaluated through laboratory tests and physical simulation experiments.The results showed that the 16S rDNA full-length sequence of the isolated strain capable of reducing Cr(Ⅵ)was analyzed in Ezbiocloud data,and the similarity was 98.89%with Desulfovibrio desulfuricans DSM 642T,named D.desulfuricans S-7.The most suitable growth temperature for D.desulfuricans S-7 was 30℃,while the most suitable pH was 7.0,and the suitable NaCl concentration was 0 to 10%.The results showed that the removal rate of 25 mg/L Cr(Ⅵ)was 79.74%,and the growth and reproduction of the strain was inhibited under an initial 100 mg/L of Cr(Ⅵ).The simulation experiment results revealed Cr(Ⅵ)concentration in the soil changed from 50.0 mg/kg to 3.1 mg/kg,and its removal rate reached 93.8%,while the remediated soil could meet China's national standard,GB 36600—2018,for Class 1 development land.This study showed that D.Desulfurians S-7 can effectively remediate Cr(Ⅵ)contaminated soil in anaerobic environment,providing a new alternative for in-situ remediation of deep-layer Cr(Ⅵ)contaminated soil.

关 键 词：硫酸盐还原菌 Desulfovibrio desulfuricans 六价铬 污染土壤 原位修复 

分 类 号：X172[环境科学与工程—环境科学] X53