一叶抗流感胶囊对人冠状病毒229E湿热疫毒袭肺小鼠药理作用研究  

Pharmacological Effect of Yiye Anti-influenza Capsule on Dampness-heat Epidemic Virus Pneumonia Mice Infected by HCoV-229E

作　　者：张敬升 赵荣华[1] 张薇 冀祖恩 庞博 徐英莉 曹姗 孙静[1] 郭姗姗[1] 彭一峰 崔晓兰[1] ZHANG Jingsheng;ZHAO Ronghua;ZHANG Wei;JI Zuen;PANG Bo;XU Yingli;CAO Shan;SUN Jing;GUO Shanshan;PENG Yifeng;CUI Xiaolan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Xinjiang Key Laboratory for Research of Licorice and Products,Korla 841011,China;Xinjiang Quanan Pharmaceutical Co.,Ltd,Korla 841011,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]新疆甘草及制品研究重点实验室,库尔勒841011 [3]新疆全安药业股份有限公司,库尔勒841011

出　　处：《病毒学报》2024年第2期233-240,共8页Chinese Journal of Virology

基　　金：国家重点研发计划(项目号:2021YFC1712903),题目:治疗新冠肺炎临床有效的宣肺败毒化湿治法的现代生物学机制研究;中国中医科学院科技创新工程(项目号:C12021A04606),题目:针对疫病早期防治的中药筛选及药效评价应急性平台建设;新疆维吾尔自治区重大科技专项(项目号:2020A03004),题目:新彊地产特色抗病毒新药创制及关键技术研究。

摘　　要：了解一叶抗流感胶囊对人冠状病毒229E(Human coronavirus 229E,HCo V-229E)湿热疫毒袭肺小鼠的药理作用,为扩展其临床应用提供参考。使用HCo V-229E滴鼻感染和湿热环境造模小鼠,考察一叶抗流感胶囊高、中、低不同剂量组[0.704g/(kg·d)、0.352g/(kg·d)、0.176g/(kg·d)三个剂量]对小鼠的肺指数、病毒载量和小鼠肺组织中IL-6、IL-10、TNF-α含量及下丘脑中PGE2、cAMP含量,同时检测小鼠外周血中T淋巴细胞亚群和B淋巴细胞比例,并设西药磷酸氯喹和中成药连花清瘟颗粒作为阳性对照药。一叶抗流感胶囊三个剂量组均可降低小鼠肺指数,高剂量组和中剂量组可降低肺炎模型小鼠的病毒载量,三个剂量组肺组织中TNF-α、IL-6及IL-10含量与模型组比较均下降,下丘脑中c AMP、PGE2含量与模型组比较均降低,且差异具有统计学意义;高、中剂量组外周血CD4+、CD8+T百分比与模型组比较升高,差异有统计学意义;高、中剂量组外周血B细胞百分比与模型组比较均下降,但差异无统计学意义。表明一叶抗流感胶囊对HCo V-229E湿热疫毒袭肺小鼠模型具有治疗作用。We investigated the pharmacological effects of Yiye anti-influenza capsule on mice with dampnessheat epidemic virus pneumonia caused by human coronavirus 229E(HCoV-229E)infection to provide a reference for its clinical application.HCoV-229E(nasal drops)was used to infect mice.The Lung Index and viral load,as well as the contents of prostaglandin(PGE)2 and cyclic adenosine monophosphate(cAMP)in the hypothalamus and contents of interleukin(IL)-6,IL-10 and tumor necrosis factor(TNF)-αin the lung tissue of mice were investigated in high-,medium-and low-dose groups of Yiye anti-influenza capsule(0.704 g/(kg·d),0.352 g/(kg·d)and 0.176 g/(kg·d)).The proportion of T-lymphocyte subsets and B lymphocytes in the peripheral blood of mice was detected,and chloroquine phosphate and Lianhua qingwen granules were used as positive controls.The three dose groups of Yiye anti-influenza capsule could reduce the Lung Index of mice.The high-dose group and medium-dose group could reduce the viral load of pneumonia-model mice.The contents of cAMP and PGE2 in the hypothalamus of mice in the three dose groups were decreased compared with those in the model group.The contents of TNF-α,IL-6 and IL-10 in lung tissue were decreased compared with those of the model group,and the difference was significant.The percentage of cluster of differentiation CD4+and CD8+T cells in peripheral blood of high-and medium-dose groups was higher than that of the model group,and the difference was significant.The percentage of B cells did not change significantly compared with that in the model group.Our results showed that Yiye anti-influenza capsule had a therapeutic effect on the mice model of pneumonia caused by HCoV-229E infection.

关 键 词：一叶抗流感胶囊 人冠状病毒229E 肺指数 病毒载量 肺组织 下丘脑 外周血 

分 类 号：R373.1[医药卫生—病原生物学]