咸阳市2018-2022年柯萨奇A组16型病毒序列分析  

Sequence Analysis of Coxsackievirus A16 in Xianyang,China from 2018 to 2022

作　　者：王士峰 秦龙 李珍妮 王丽娟 张丹霞 蒋宇婷 WANG Shifeng;QIN Long;LI Zhenni;WANG Lijuan;ZHANG Danxia;JIANG Yuting(Xianyang Center for Disease Control and Prevention,Xianyang 712000,China)

机构地区：[1]咸阳市疾病预防控制中心,咸阳712000

出　　处：《病毒学报》2024年第2期418-423,共6页Chinese Journal of Virology

摘　　要：本研究旨在了解咸阳市2018-2022年手足口病(Hand, foot and mouth disease, HFMD)病原柯萨奇A组16型(Coxsackievirus A16, CV-A16)基因进化特征,为HFMD防控策略制定提供依据。首先分析2018-2022年咸阳市HFMD监测数据,再选取部分CV-A16阳性样本进行RT-PCR扩增VP1基因序列,测序后进行系统进化和序列相似性分析。2018-2022年咸阳市CV-A16阳性确诊病例共计402例,占实验室确诊病例总数的28.69%(402/1401)。VP1序列系统进化分析显示选取的54条CV-A16 VP1均为B1基因亚型,25条属B1a型,29条属B1b型。2018-2020年以B1b型为主,而2021-2022年主要为B1a型。氨基酸序列相似性分析表明,B1a型序列存在T164K(25/25)、I251V(25/25)和A22T(3/25)三处突变,而B1b型主要有N14S(9/29)和I235V(6/29)突变。CV-A16为咸阳市2018年、2019年、2021年和2022年HFMD主要致病原之一,2021年后优势毒株可能已由B1b型转化为B1a型,将来应加强CV-A16基因型动态监测。This study was aimed to investigate the genetic evolutionary characteristics of coxsackievirus A16(CV-A16)associated hand,foot and mouth disease(HFMD)in Xianyang,China from 2018 to 2022 and to facilitate the establishment of strategies for HFMD control.Survellance data of HFMD samples in Xianyang,China from 2018 to 2022 were analyzed,then the viral capsid protein 1(VP1)sequences of selected samples were amplified with RT-PCR and sequenced.Full VP1 sequences were determined to create the phylogeny and sequence similarity analysis.During 2018-2022,a total of 402 CV-A16 positive cases were identified,accounting for 28.69%(402/1401)of the laboratory-confirmed cases.VP1-based phylogenetic analysis revealed that 54 sequences belonged to sub-genotype B1,in which 25 belonged to Bla evolutionary branches and 29 belonged to B1b.During 2018-2020 B1b was the major strain,while Bla has become predominant from 2021.Amino acid sequence analysis showed that the Bla contained T164K(25/25),I251V(25/25)and A22T(3/25)mutations,while B1b mainly contained N14S(9/29)and I235V(6/29).CV-A16 was one of the major pathogens of HFMD in Xianyang,China in 2018,2019,2021 and 2022,the dominant strain in Xianyang,China may have converted from Blb to Bla since 2021.The dynamic monitoring of CV-A16 genotypes should be strengthened.

关 键 词：手足口病 柯萨奇病毒A组16型 VP1序列 进化分析 

分 类 号：R373.2[医药卫生—病原生物学]