应当引起关注的昆虫细胞中的弹状病毒污染  

Rhabdovirus Contamination in Insect Cells Should be of Concern

作　　者：魏佳琪 孙振 薛秀 孟子燕 于馨 刘洋 张建龙 姜琳琳[1] 张兴晓 朱洪伟 于佳玉 WEI Jiaqi;SUN Zhen;XUE Xiu;MENG Ziyan;YU Xin;LIU Yang;ZHANG Jianlong;JIANG Linlin;ZHANG Xingxiao;ZHU Hongwei;YU Jiayu(College of Life Science,Ludong University,Yantai 264025,China)

机构地区：[1]鲁东大学生命科学学院,烟台264025

出　　处：《病毒学报》2024年第2期432-437,共6页Chinese Journal of Virology

基　　金：烟台市校地融合发展项目:新型宠物疫苗研发与成果转化平台。

摘　　要：Sf弹状病毒(Sf-rhabdovirus,Sf-RV)是2014年从雌性草地贪夜蛾(Spodoptera frugiperda,Sf)蛹中分离得到的昆虫细胞系(Sf)中发现的一种弹状病毒,为线性负义单链RNA病毒。在Sf细胞系中发现的新弹状病毒(Sf-RV)使人们对昆虫细胞杆状病毒表达载体系统(Insect cell baculovirus expression vector system,IC-BEVS)生产的生物制剂的安全性表示质疑。本文主要对该病毒基因组构成、组成蛋白功能、感染宿主细胞能力宿主细胞对病毒的影响以及病毒带来的潜在安全性问题等方面进行简要阐述,以增加人们的认识。Sf-rhabdovirus(Sf-RV),a linear negative-sense single-stranded RNA virus,was found in insect cell line(Sf)isolated from female Armyworm(Spodoptera frugiperda)larvae in 2014.The discovery of Sfrhabdovirus(Sf-RV)in the Sf cell line raises questions about the safety of biologics produced by the insect cell baculovirus expression vector system(IC-BEVS).In this paper,the genome structure,the function of component proteins,the ability to infect host cells,the influence of host cells on the virus and the potential safety problems brought by the virus were briefly described,so as to increase people's understanding of the new rhabdovirus.

关 键 词：Sf弹状病毒 Sf细胞系 昆虫细胞杆状病毒表达载体系统 

分 类 号：Q93[生物学—微生物学]