核桃组培苗对黑斑病菌侵染的响应  

Response of Walnut Tissue Cultures to Blight Bacteria Infection

作　　者：安秀红 王宁 陈弈可 张文龙 王红霞[1] AN Xiuhong;WANG Ning;CHEN Yike;ZHANG Wenlong;WANG Hongxia(Mountainous Areas Research Institute,Hebei Agricultural University/Technology Innovation Center for Agriculture in Mountainous Areas of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding,Hebei 071001)

机构地区：[1]河北农业大学河北省山区研究所,河北省山区农业技术创新中心,国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《北方园艺》2024年第7期27-34,共8页Northern Horticulture

基　　金：“十四五”国家重点研发计划资助项目(2022YFD2200402);河北省重点研发计划资助项目(21326352D);河北农业大学引进人才科研专项资助项目(YJ2021006)。

摘　　要：以“清香”和“赞美”核桃组培苗为试材,采用茎部伤口浸泡接种的方法进行病菌接种,研究了黑斑病致病菌成团泛菌对核桃生理生化的影响,以期为核桃黑斑病防治提供参考依据。结果表明:黑斑病菌接种降低了2个核桃品种组培苗中叶绿素、可溶性糖含量及光合能力。随着病菌接种,2个核桃品种中超氧化物歧化酶(SOD)活性增强,过氧化物酶(POD)和过氧化氢酶(CAT)活性在接种初期变化相反,之后变化趋势相同。2个品种中总酚含量先升高后降低;“清香”中类黄酮含量在病菌接种初期增多,之后降低,而“赞美”中类黄酮含量变化幅度较小。植物激素在病菌接种后变化差异较大,生长素(IAA)和玉米素(ZT)在“清香”中含量增多,“赞美”中先降低后升高;激动素(KT)含量在“清香”中增多,“赞美”中无显著差异;赤酶素(GA)含量在2个品种中均增多;脱落酸(ABA)含量在“清香”中降低,而“赞美”中先升高后降低;水杨酸(SA)含量在“清香”中呈先升高后降低再升高的趋势,而“赞美”中含量不断降低。该研究初步确定组培苗内总酚、类黄酮和植物激素IAA、ZT、SA等含量的增多与黑斑病抗性密切相关。‘Qingxiang’and‘Zanmei’walnut tissue cultures were used as experimental materials and were inoculated with Pantoea agglomerans by stem wound immersion.The effects of walnut blight bacteria on the physiological and biochemical changes of the two varieties were studied,in order to provide reference for the control of walnut blight bacteria.The results showed that the contents of chlorophyll and soluble sugar and photosynthetic capacity in‘Qingxiang’and‘Zanmei’tissue cultures were decreased after bacteria inoculation.SOD activity increased in both varieties,while POD and CAT activities showed opposite changes at the beginning of inoculation and then showed the same trend.The total phenol content of the two varieties increased first and then decreased.At the beginning of inoculation,the content of flavonoids in‘Qingxiang’first increased and then decreased,while the flavonoid content in‘Zanmei’changed little.The changes of plant hormones were different after inoculation.The contents of IAA and ZT increased in‘Qingxiang’,and first decreased and then increased in‘Zanmei’.KT content increased in‘Qingxiang’,but there was no significant difference in‘Zanmei’.GA content increased in both varieties.ABA content decreased in‘Qingxiang’but increased first and then decreased in‘Zanmei’.SA content in‘Qingxiang’increased first,then decreased,and then increased,while the content in‘Zanmei’decreased continuously.This study preliminarily determined that the increase of total polyphenols,flavonoids,plant hormones IAA,ZT and SA in tissue cultures was closely related to the resistance of walnut blight.

关 键 词：核桃 黑斑病菌 抗氧化物酶活性 次生代谢物质 植物激素 

分 类 号：S664.1[农业科学—果树学]