外源硒对鸡腿菇菌丝生长及合成主要营养素的影响  

Effects of Sodium Selenite on the Growth and Synthesis of Major Nutrients in the Mycelium of Coprinus comatus

作　　者：唐茂林 张洋[1] 王亚艺[1] 李铂坤 李松龄[1] TANG Maolin;ZHANG Yang;WANG Yayi;LI Bokun;LI Songling(Academy of Agriculture and Forestry Sciences,Qinghai University,Xining,Qinghai 810016)

机构地区：[1]青海大学农林科学院,青海西宁810016

出　　处：《北方园艺》2024年第7期112-119,共8页Northern Horticulture

基　　金：青海省农林科学院创新基金资助项目(2023-NKY-01);青海省科技特派员专项资助项目(2023-NK-P30)。

摘　　要：以1株鸡腿菇菌株Cc-900为试材,采用平板培养和液体发酵培养的方法,研究不同浓度的外源硒对鸡腿菇菌株Cc-900的生长、富硒效果和菌丝合成蛋白质和多糖的影响,以期为富硒鸡腿菇的栽培和产品的开发提供参考依据。结果表明:鸡腿菇菌丝可耐受10 mg·kg^(-1)硒浓度的固体培养基和最大硒浓度为3 mg·L^(-1)的液体培养基,高浓度硒对菌丝活性损伤较大;当液体培养基中硒浓度为3 mg·L^(-1)时,菌丝干质量可达1.97 g·L^(-1),菌丝干粉的硒含量为38.24 mg·kg^(-1),其硒富集系数可达12.75,菌丝中粗蛋白和多糖含量分别为2.58%和3 081.82μg·g^(-1),发酵液中可溶性蛋白质和多糖含量分别为50.98μg·mL^(-1)和266.38μg·mL^(-1)。液体发酵培养中硒浓度在3 mg·L^(-1)以下可促进鸡腿菇菌丝的生长和硒的富集,同时促进菌丝中蛋白质的合成和释放,但会抑制菌丝中多糖的合成。Taking a Coprinus comatus strain Cc-900 as the experimental material,plate culture and liquid fermentation culture were used to study the effects of different concentrations of exogenous selenium on the growth,selenium enrichment,and protein and polysaccharide synthesis of a Coprinus comatus strain Cc-900,in order to provide reference for the cultivation and product development of Coprinus comatus.The results showed that the optimal tolerance of Coprinus comatus mycelium to selenium concentration was at 10 mg·kg^(-1) in solid culture medium and 3 mg·L^(-1) in liquid culture medium,whereas high concentrations of selenium caused reduction of mycelial activity and significant damage to mycelial growth.On the other hand,1.97 g·L^(-1) mycelium dry weight,38.24 mg·kg^(-1) selenium content of the mycelium powder,and a selenium enrichment coefficient at 12.75 were achieved at 3 mg·L^(-1) selenium in liquid culture medium.Likewise,crude protein and polysaccharide contents in the mycelium was 2.58%and 3081.82μg·g^(-1),respectively,while soluble protein and polysaccharide contents in the fermentation broth was 50.98μg·mL^(-1) and 266.38μg·mL^(-1),respectively.The results showed that Selenium concentrations below 3 mg·L^(-1) in liquid fermentation culture can promote the growth and selenium enrichment of Coprinus comatus hyphae,as well as the synthesis and release of proteins,while inhibiting the synthesis of polysaccharides.

关 键 词：鸡腿菇 亚硒酸钠 富硒 蛋白质 多糖 

分 类 号：S646.9[农业科学—蔬菜学]