化浊解毒颗粒对脂多糖所致的急性肺损伤大鼠炎症相关指标的影响  

Effect of Huazhuo Jiedu Granules on Inflammation Related Indicators in Rats with Acute Lung Injury Caused by Lipopolysaccharide

作　　者：董琛 张欢欢 高芳芳 韩世强 刘伟花 武蕾[2] 景璇[2] 贾琳[2] DONG Chen;ZHANG Huan-huan;GAO Fang-fang;HAN Shi-qiang;LIU Wei-hua;WU Lei;JING Xuan;JIA Lin(Hebei University of Chinese Medicine,Shijiazhuang 050091)

机构地区：[1]河北中医药大学,石家庄050091 [2]河北省中医院,石家庄050013 [3]石家庄市中医院,石家庄050011

出　　处：《河北中医药学报》2024年第2期7-12,共6页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省重点研发计划项目:No.21377723D。

摘　　要：目的:探讨化浊解毒颗粒对急性肺损伤(Acute lung injury, ALI)大鼠炎症相关指标的影响。方法:60只大鼠随机分为空白组,模型组,泼尼松组,化浊解毒低、中、高剂量组。除空白组外,其余各组给药7 d后构建ALI模型。行肺组织病理学观察及肺损伤评分,测定肺组织湿/干重比(W/D)、肺通透性指数、动脉血氧分压(PaO_(2))、肺泡灌洗液(BALF)炎性细胞分类计数,肺组织核因子κB(NF-κB)p65、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素10(IL-10)mRNA表达,并检测肺组织白细胞介素1β(IL-1β)、巨噬细胞炎性蛋白1a(MIP-1a)含量。结果:与空白组比较,模型组大鼠肺组织结构紊乱,间质水肿,炎性细胞浸润;同时肺损伤评分、肺组织W/D、肺通透性指数、BALF炎性细胞数目,肺组织NF-κB p65、TNF-α、IL-6 mRNA表达,IL-1β、MIP-1a水平均升高(P<0.05),PaO_(2)、IL-10 mRNA表达均降低(P<0.05)。与模型组相比,化浊解毒低、中、高剂量组肺组织炎性细胞浸润及水肿不同程度减轻,且肺损伤评分、肺组织W/D、肺通透性指数、BALF炎性细胞数目、肺组织NF-κB p65 mRNA表达、IL-1β、MIP-1a水平均降低(P<0.05),PaO_(2)升高(P<0.05);化浊解毒中、高剂量组肺组织IL-6和TNF-α mRNA表达降低(P<0.05),IL-10 mRNA表达升高(P<0.05)。结论:化浊解毒颗粒能改善ALI模型大鼠肺组织病理损伤,其机制可能与调控肺内炎症因子水平有关。Objective:To explore the effect of Huazhuo Jiedu Granules(granules for eliminating turbid and removing toxin)on in-flammation related indicators in rats with acute lung injury(ALI)Methods:Sixty rats were divided into blank group,model group,prednisone group,low-,medium-,and high-dose groups of Huazhuo Jiedu Granules After 7 days of administration,ALI model rats were constructed Observation of lung tissue pathology and lung injury score were used to measure lung tissue wet/dry weight ratio(W/D),lung permeability index,arterial partial pressure of oxygen(PaO_(2)),classification count of inflammatory cells in bronchoalveolar lavage fluid(BALF),lung tissue nuclear factorκB(NF-κB)P65,Tumor Necrosis Factorα(TNF-α),mRNA expression of inter-leukin-6(IL-6)and interleukin-10(IL-10),and to detect interleukin-1β(IL-1β)in lung tissue and macrophage inflammatory pro-tein-1a(MIP-1a)Results:Compared with the blank group,the lung tissue structure of the model group was disordered,with intersti-tial edema and inflammatory cell infiltration;At the same time,lung injury score,lung tissue W/D,lung permeability index,BALF inflammatory cell count increased(P<005),PaO_(2) decreased(P<005),and NF-κB p65,TNF-α,IL-6 mRNA expression in-creased(P<005),IL-10 mRNA expression decreased(P<005),IL-1βand MIP-1a increased(P<005)Compared with the model group,the low-,medium-,and high-dose groups of Huazhuo Jiedu Granules showed that inflammatory cell infiltration and edema in lung tissue in varying degrees,and lung injury score,lung tissue W/D,lung permeability index,and BALF inflammatory cell count decreased(P<005),PaO_(2)(P<005)increased,and mRNA expression of NF-κB p65 in lung tissue reduced(P<005),IL-1βand MIP-1a decreased(P<005);mRNA expression of IL-6 and TNF-αMedium-and high-dose groups decreased(P<005),while IL-10 mRNA expression increased(P<005)Conclusion:Huazhuo Jiedu Granules can improve the pathological damage of lung tissue in ALI model rats,and its mechanism may be related to regulating the levels of inflammatory factors in

关 键 词：急性肺损伤 化浊解毒 NF-ΚB 炎症因子 脂多糖 

分 类 号：R285.5[医药卫生—中药学]