禾肾丸通过调控ANGPTL3治疗阿霉素肾病大鼠蛋白尿及血脂代谢紊乱的实验研究  

Experimental study of Heshen pill on the treatment of proteinuria and dyslipidemiain rats with adriamycin nephropathy through the regulation of ANGPTL3

作　　者：何怡然 欧阳林旗[1] 徐文峰[2] 郭宇鸽[1] 肖望重[1] 黎旭 HE Yiran;OUYANG Linqi;XU Wenfeng;GUO Yuge;XIAO Wangzhong;LI Xu(Department of Pharmacy,The First Affiliated Hospital of Hunan Chinese Medicine University,Changsha Hunan 410013,China;Department of Nephrology,The First Affiliated Hospital of Hunan Chinese Medicine University,Changsha Hunan 410013,China)

机构地区：[1]湖南中医药大学第一附属医院药学部,湖南长沙410013 [2]湖南中医药大学第一附属医院肾内科,湖南长沙410013

出　　处：《蚌埠医学院学报》2024年第3期286-290,共5页Journal of Bengbu Medical College

基　　金：湖南省卫生健康委科研课题(202103050979);湖南中医药大学校级科研基金项目(2019XJJJ055)。

摘　　要：目的:探讨禾肾丸通过调控血管生成素样蛋白3(ANGPTL3)治疗阿霉素肾病大鼠蛋白尿及血脂代谢紊乱的作用机制。方法:50只雄性SD大鼠随机分为5组,每组10只,空白组:不作特殊处理,仅予以每日清晨蒸馏水灌胃;模型组:造模成功后每日清晨蒸馏水灌胃;禾肾丸组:造模成功后每日清晨以禾肾丸灌胃;对照组:造模成功后每日清晨以泼尼松灌胃;联合用药组:造模成功后每日清晨以泼尼松、禾肾丸灌胃,各组均灌胃8周。密切观察各组大鼠造模及研究期间各种行为学改变,检测和比较各组大鼠造模前1 d及造模后第2、4、6、8周末24 h尿蛋白水平,同时检测和比较各组大鼠血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)等血脂指标及血清ANGPTL3表达水平。分析各组大鼠血清ANGPTL3表达水平与血脂指标及造模后第8周末24 h尿蛋白水平的相关性。结果:禾肾丸组、对照组及联合用药组大鼠造模后第8周各种行为学改变均优于模型组;各组大鼠造模前1 d 24 h尿蛋白水平比较差异无统计学意义(P>0.05),造模后第2、4周模型组、禾肾丸组、对照组及联合用药组大鼠24 h尿蛋白水平均明显高于空白组(P<0.05),而组间比较差异无统计学意义(P>0.05),造模后第6、8周禾肾丸组、对照组大鼠24 h尿蛋白水平明显高于联合用药组和低于模型组(P<0.05),而禾肾丸组与对照组比较差异无统计学意义(P>0.05);模型组、禾肾丸组、对照组及联合用药组大鼠TC、TG、LDL-C等血脂指标及血清ANGPTL3表达水平均明显高于空白组(P<0.05),而HDL均明显低于空白组(P<0.05),且禾肾丸组、对照组大鼠TC、TG、LDL-C及ANGPTL3均低于模型组和高于联合用药组(P<0.05),HDL-C均高于模型组和低于联合用药组(P<0.05),而禾肾丸组与对照组比较差异无统计学意义(P>0.05);各组大鼠血清ANGPTL3表达水平与TC、TG、LDL-C及24 h尿蛋Objective:To investigate the mechanism of Heshen pill in the treatment of proteinuria and dyslipidemia in adriamycin nephropathy rats through the regulation of angiopogenin-like protein 3(ANGPTL3).Methods:Fifty male SD rats were randomly divided into 5 groups with 10 rats in each group.The blank group was given distilled water by gavage every morning without special treatment;the model group was given distilled water by gavage every morning after successful modeling;the Heshen pill group was given Heshen pill by gavage every morning after successful modeling;the control group was given prednisone by gavage every morning after successful modeling;the combined treatment group was given prednisone and Heshen pill by gavage every morning after successful modeling.Each group was gavaged for 8 weeks.The behavioral changes of rats in each group were closely observed during the period of modeling and the research.The levels of 24 h urinary protein at 1 day before modeling and at the end of 2,4,6,and 8 weeks after modeling were detected and compared.At the same time,the various serum lipid indexes such as TC,TG,LDL-C,HDL-C,and serum expression level of ANGPTL3 were detected and compared.The correlation between serum ANGPTL3 expression level and blood lipid indexes,24 h urinary protein level at the end of the eighth week after modeling was analyzed.Results:All behavioral changes of rats in Heshen pill group,control group,and combined treatment group were better than that in model group at 8 weeks after modeling.There was no significant difference in the 24 h urinary protein level before 1 d of modeling among all groups(P>0.05).The 24 h urinary protein levels in the model group,Heshen pill group,control group,and combined treatment group were all significantly higher than that in blank group at 2 and 4 weeks after modeling(P<0.05),while there was no significant difference among each group(P>0.05).The 24 h urinary protein levels in Heshen pill group and control group were significantly higher than that in combined treatment

关 键 词：肾病综合征 血脂代谢紊乱 蛋白尿 禾肾丸 血管生成素样蛋白3 阿霉素肾病大鼠 

分 类 号：R285.5[医药卫生—中药学]