桑叶中噻嗪酮和三环唑残留检测研究  

Study on the detection of buprofezin and tricyclazole residues in mulberry leaf

作　　者：李国烈 LI Guolie(Nanchong Monitoring and Test Center for Agricultural Products Quality,Sichuan Nanchong637000,China)

机构地区：[1]南充农产品质量监测检验中心,四川南充637000

出　　处：《现代农药》2024年第2期75-79,共5页MODERN AGROCHEMICALS

摘　　要：建立超高效液相色谱-串联质谱方法检测桑叶中噻嗪酮和三环唑残留。样品经乙腈提取,石墨化炭黑氨基复合柱(Carbon/NH2)净化,在电喷雾离子源正离子多反应监测模式下检测,基质外标法定量。噻嗪酮和三环唑的定量限均为0.001 mg/kg,在0.000 1~0.10 mg/L质量浓度范围内线性关系良好,相关系数大于0.999。在0.001~0.10 mg/kg质量分数范围内,平均回收率为91.7%~104.5%,方法精密度相对标准偏差为1.31%~8.46%。该方法灵敏度高,准确度好,适用于桑叶中噻嗪酮和三环唑残留量的测定。An ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for quantification of buprofezin and tricyclazole in mulberry leaves was developed.The samples were extracted with acetonitrile,purified by Carbon/NH2,detected under multiple reaction monitoring mode with positive electrospray ionization,and quantified by the method of matrix matched external standard.The results showed that the limits of quantification for buprofezin and tricyclazole were 0.001 mg/kg.The linear relationship was good at the concentrations of 0.0001-0.10 mg/L,and the correlation coefficients were over 0.999.With the addition concentration of 0.001-0.10 mg/kg,the average recoveries ranged from 91.7% to 104.5%,and the relative standard deviations were 1.31%-8.46%.The method was sensitive and accurate for the determination of buprofezin and tricyclazole residues in mulberry leaves.

关 键 词：桑叶 噻嗪酮 三环唑 残留 基质效应 超高效液相色谱-串联质谱 

分 类 号：TQ450.263[化学工程—农药化工]