髓过氧化物酶-463G/A基因多态性与彝族原发性高血压的相关性  

Association between Myeloperoxidase-463G/A Gene Polymorphism and Essential Hypertension in Yi Nationality

作　　者：马承娅 杨江 陈曦 徐茜 尹丕轩 舒芯 彭红瑜 范志祥[2] 龙莉[2] MA Chengya;YANG Jiang;CHEN Xi;XU Xi;YIN Pixuan;SHU Xin;PENG Hongyu;FAN Zhixiang;LONG Li(School of Basic Medicine,Kunming Medical University,Kunming Yunnan 650500,China;Dept.of Cell Biology and Medical Genetics,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学基础医学院,云南昆明650500 [2]昆明医科大学细胞生物学和医学遗传学系,云南昆明650500

出　　处：《昆明医科大学学报》2024年第4期57-61,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2019FE001-021,2018FE001(-188)];昆明医科大学大学生创新性实验计划基金资助项目(2022JXD300)。

摘　　要：目的研究髓过氧化物酶(MPO)-463G/A基因的多态性与云南彝族原发性高血压(EH)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RELP)技术,检测2007年1月至2008年11月由昆明医科大学第一附属医院采自云南省双河乡彝族高血压患者(EH组,n=86)和彝族正常人(对照组,n=100)的MPO-463G/A基因多态性,并计算GG、GA、AA基因型频率及G、A基因频率,再进行关联分析。结果在EH组和对照组中,MPO基因-463G/A多态的基因型频率GG为66.28%、52.00%,GA+AA为33.72%、48.00%,等位基因G频率为79.65%、69.50%,等位基因A频率为20.35%、30.50%,2组基因型频率和基因频率分别相比,差异均具有统计学意义(P<0.05)。彝族女性EH组和女性对照组中GG和GA+AA基因型频率分别为74.50%、49.30%和25.50%、50.70%,等位基因G和A的频率分别为85.50%、69.00%和14.50%、31.00%,2组基因型频率和基因频率分别相比,差异均具有统计学意义(P<0.05),EH组A基因频率明显低于对照组。彝族男性EH组和男性对照组的基因型频率和基因频率分别比较,差异均无统计学意义(P>0.05)。结论MPO基因-463G/A多态与云南彝族女性原发性高血压具有相关性,是彝族女性高血压的易感基因。该位点A基因的表达在彝族女性EH的发生过程中起保护性作用。Objective To study the correlation between the polymorphism of myeloperoxidase-463G/A gene and essential hypertension of Yi nationality in Yunnan Province.Methods The PCR-RFLP technique was used to detect the MPO-463G/A gene polymorphism in 86 Yi hypertensive patients and 100 normal Yi people in Shuanghe Township and Yunnan Province collected by the First Affiliated Hospital of Kunming Medical University from January 2007 to November 2008.The frequencies of the GG,GA,and AA genotypes and the G and A allele frequencies were calculated,followed by association analysis.Results The genotype frequencies of MPO gene-463G/A polymorphism in EH group and control group GG were 66.28%,52.00%,GA+AA were 33.72%,48.00%,respectively.The frequencies of allele G were 79.65%and 69.50%,and the frequencies of allele A were 20.35%and 30.50%,respectively.There were statistically significant differences in genotype frequency and gene frequency between the two groups(P<0.05).The genotype frequencies of GG and GA+AA were 74.50%and 49.30%in female EH group and 25.50%and 50.70%in femal control group.The allele frequencies of G and A were 85.50%and 69.00%in EH group and 14.50%and 31.00%in control group.The differences were statistically significant(P<0.05).The frequency of A gene in female EH group was significantly lower than that in female control group.There was no significant difference in genotype frequency and gene frequency between EH group and control group in Yi male(P>0.05).Conclusion MPO gene-463G/A polymorphism is related to essential hypertension in Yunnan Yi women and is a susceptibility gene for hypertension in Yi women.The expression of the A allele at this locus has a protective effect during the development of EH in Yi women.

关 键 词：髓过氧化物酶 原发性高血压 彝族 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]