大黄牡丹汤治疗脓毒症胃肠功能障碍作用机制的网络药理学研究  

Mechanism of Dahuang Mudan Decoction in Treating Gastrointestinal Dysfunction in Sepsis

作　　者：杨梦霞 武浩文 赵春铭 陈腾飞[1] 徐霄龙[1,4] 刘清泉 Yang Mengxia;Wu Haowen;Zhao Chunming;Chen Tengfei;Xu Xiaolong;Liu Qingquan(Beijing Hospital of Traditional Chinese Medicine of Capital Medical University,University,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京中医药大学,北京100029 [3]河北工程大学,河北邯郸056038 [4]首都医科大学中医脓毒症临床诊疗与研究中心,北京100010

出　　处：《中国中医急症》2024年第4期582-586,600,共6页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023001)。

摘　　要：目的通过网络药理学方法探寻大黄牡丹汤治疗脓毒症胃肠功能障碍的潜在分子作用机制。方法从TCMSP、TCMID和TCM Database@Taiwan中药数据库获取并筛选大黄牡丹汤化学成分,并利用Swiss Target Prediction数据库检索药物作用靶点;使用GeneCards筛选脓毒症和胃肠功能障碍的疾病相关作用位点;利用Venny2.1.0在线工具获取药物作用靶点和疾病相关作用位点的交集基因,并结合STRING12.0数据库和Cytoscape3.9.1软件绘制多层次相互作用的“中药-化合物-潜在治疗靶点”网络图;运用DAVID数据库完成对潜在治疗靶点的GO和KEGG通路富集分析。结果共得到大黄牡丹汤的53个化学成分及其479个作用靶点,与2121个脓毒症相关作用位点、6785个胃肠功能障碍相关作用位点取交集后得到124个潜在治疗靶点。大黄牡丹汤治疗脓毒症胃肠功能障碍的关键靶点为白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)及Akt1等。通过富集分析发现,大黄牡丹汤治疗脓毒症胃肠功能障碍是通过调控HIF-1信号通路、PI3K-Akt信号通路及白细胞介素-17(IL-17)信号通路等发挥抗炎、调节免疫系统的作用。结论通过网络药理学方法发现大黄牡丹汤治疗脓毒症胃肠功能障碍的作用机制复杂,涉及多成分、多靶点和多通路,可能与抗炎、免疫系统等相关。Objective:To explore the potential molecular mechanism of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis through network pharmacology.Methods:The chemical components of Dahuang Mudan Decoction were obtained and screened from TCMSP,TCMID and TCM Database@Taiwan database,and the drug targets were retrieved using Swiss Target Prediction database;Genecards was used to screen the disease-related action sites of sepsis and gastrointestinal dysfunction;the intersection genes of drug action targets and disease-related action sites were obtained by Venny 2.1.0 online tool,and the network diagram of“traditional Chinese medicine-compounds-potential therapeutic targets”with multi-level interaction was drawn by combining string 12.0 database and Cytoscape 3.9.1 software;David database was used to complete the GO and KEGG pathway enrichment analysis of potential therapeutic targets.Results:A total of 53 chemical components and 479 action targets of Dahuang Mudan Decoction were obtained,and 124 potential therapeutic targets were obtained after intersection with 2121 sepsis-related action sites and 6785 gastrointestinal dysfunction-related action sites.The key targets of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis were IL-6,IL-1β,Akt1,etc.Through enrichment analysis,it was found that Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis played an anti-inflammatory and immune system regulating role by regulating HIF-1 signaling pathway,PI3K-Akt signaling pathway,IL-17 signaling pathway,etc.Conclusions:Through the network pharmacology method,we found that the mechanism of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis is complex,involving multiple components,multiple targets and multiple pathways,which may be related to anti-inflammatory,immune system,etc.

关 键 词：脓毒症 胃肠功能障碍 大黄牡丹汤 网络药理学 分子机制 

分 类 号：R631[医药卫生—外科学]