桑白皮汤含药血清调控人支气管上皮细胞黏液高分泌的机制研究  

Study on the Mechanism of Sangbaipi Decoction Containing Serum in Regulating Mucus Hypersecretion in Human Bronchial Epithelial Cells

作　　者：谭卫 刘雨 张钰 李福星 彭朗萍 章澳 谭光波 Tan Wei;Liu Yu;Zhang Yu;Li Fuxing;Peng Langping;Zhang Ao;Tan Guangbo(Hunan University of Traditional Chinese Medicine,Hunan,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医研究院,湖南长沙412000 [3]湖南省中医药研究院附属医院,湖南长沙412000

出　　处：《中国中医急症》2024年第4期587-590,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82004306);湖南省中医药“神农人才”工程。

摘　　要：目的探究桑白皮汤调控人支气管上皮细胞黏液高分泌的机制。方法制备桑白皮汤含药血清和乙酰半胱氨酸溶液,采用15%香烟溶液和20 ng/mL脂多糖溶液对人支气管上皮细胞进行气道黏液高分泌造模,按照药物干预分为桑白皮汤组、乙酰半胱氨酸组、空白组及模型组,采用CCK-8法检测人支气管上皮细胞活性、电子显微镜下观察细胞形态及WB法检测数据挖掘中预测的白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)蛋白表达。结果实验显示,模型组与空白组对比,细胞活性降低,桑白皮汤组与模型组对比,细胞活性升高(P<0.05)。模型组与空白组对比,炎症细胞因子IL-1β、TNF-α蛋白表达明显增多(P<0.05),表明气道黏液高分泌造模成功。桑白皮汤组、乙酰半胱氨酸组与模型组对比,IL-1β、TNF-α蛋白表达明显降低(P<0.05)。结论桑白皮汤含药血清可显著降低IL-1β、TNF-α蛋白表达,其治疗气道黏液高分泌的机制与调控炎症因子IL-1β、TNF-α有关。Objective:To explore the mechanism of Sangbaipi Decoction regulating mucus hypersecretion in human bronchial epithelial cells.Methods:Sangbaipi Decoction containing serum and acetylcysteine solution were prepared.Human bronchial epithelial cells were treated with 15%cigarette solution and 20 ng/mL lipopolysaccharide solution to establish airway mucus hypersecretion model.According to drug intervention,they were divided into Sangbaipi Decoction group,acetylcysteine group,blank group and model group.CCK-8 method was used to detect the activity of human bronchial epithelial cells,electron microscope was used to observe the morphology of cells,and WB method was used to detect the protein expression of IL-1βand TNF-a predicted by data mining.Results:The experiment showed that the cell activity was decreased in the model group compared with the blank group,and the cell activity was increased in the Sangbaipi Decoction group compared with the model group(P<0.05).The protein expression of inflammatory cytokines IL-1βand TNF-αwas significantly increased in the model group compared with the blank group(P<0.05),indicating that the airway mucus hypersecretion model was successfully established.The protein expression of IL-1βand TNF-αwas significantly decreased in the Sangbaipi Decoction group and the acetylcysteine group compared with the model group(P<0.05).Conclusion:Sangbaipi Decoction containing serum can significantly reduce the protein expression of IL-1βand TNF-α,and its mechanism of treating airway mucus hypersecretion is related to the regulation of inflammatory cytokines IL-1βand TNF-α.

关 键 词：气道黏液高分泌 桑白皮汤 人支气管上皮细胞 炎症因子 

分 类 号：R563[医药卫生—呼吸系统]