High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT  

作　　者：Joseph Rufo Peiran Zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen Ruoyu Zhong Ke Jin Ye He Jianping Xia Ke Li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee Tony Jun Huang 

机构地区：[1]Department of Mechanical Engineering and Materials Science,Duke University,Durham,NC,USA [2]Department of Biomedical Engineering,Duke University,Durham,NC,USA [3]Department of Obstetrics,Gynecology,and Reproductive Sciences,Magee-Womens Research Institute,University of Pittsburgh,Pittsburgh,PA,USA [4]Department of Microbiology and Molecular Genetics,School of Medicine,University of Pittsburgh,Pittsburgh,PA,USA [5]Renal Division and Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Boston,MA,USA [6]Department of Bioengineering,Department of Electrical Engineering and Computer Science,University of California,Berkeley,Berkeley,CA,USA [7]Department of Biophysics,Institute of Quantum Biophysics,Sungkyunkwan University,Suwon,Korea

出　　处：《Microsystems & Nanoengineering》2024年第1期313-323,共11页微系统与纳米工程（英文）

基　　金：supported by the National Institutes of Health(R01HD103727,UH3TR002978,U18TR003778,R01GM141055,R01GM132603,R01GM145960,R01GM144417,and R01GM135486);the National Science Foundation(CMMI-2104295);the National Science Foundation Graduate Research Fellowship(2139754).

摘　　要：Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various diseases.However,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low purities.Here,we report a high-yield(>90%)and rapid(<10 min)EV isolation method called FLocculation via Orbital Acoustic Trapping(FLOAT).The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid droplets.By adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the droplet.We demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid biopsies.Due to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关 键 词：TRAPPING utilize SEPARATING 

分 类 号：TB383[一般工业技术—材料科学与工程]