鸢尾素抑制炎症因子表达促成骨分化改善糖尿病小鼠骨代谢的研究  

Irisin promotes osteogenic differentiation and ameliorates bone metabolism in diabetic mice by inhibiting inflammatory factors

作　　者：田峰 涂来勇 张恩丰[1,2,3] 顾文飞 单烁 马志杰[1,2,3] 刘毅 胡铜 赵疆 TIAN Feng;TU Laiyong;ZHANG Enfeng;GU Wenfei;SHAN Shuo;MA Zhijie;LIU Yi;HU Tong;ZHAO Jiang(Hospital of Traditional Chinese Medicine of Xinjiang of Uygur Autonomous Region,Urumqi 830000,China;Xinjiang Medical University,Urumqi 830000,China;Xinjiang Academy of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区中医医院,新疆乌鲁木齐830000 [2]新疆医科大学,新疆乌鲁木齐830000 [3]新疆维吾尔自治区中医药研究院,新疆乌鲁木齐830000

出　　处：《中国骨质疏松杂志》2024年第4期511-516,共6页Chinese Journal of Osteoporosis

基　　金：新疆维吾尔自治区自然科学基金面上项目(2021D01C213)。

摘　　要：目的探讨鸢尾素在治疗糖尿病性骨质疏松模型小鼠中的疗效以及相关机制。方法基于6周龄C57BL/6小鼠构建糖尿病性骨质疏松模型小鼠,腹腔注射鸢尾素干预模型小鼠,评估其对小鼠体重、空腹血糖、糖耐量试验等指标的影响,收集小鼠血清检测鸢尾素、CTX、P1NP指标,收集骨组织及骨髓间充质干细胞并检测mRNA Runx2、IL-1β、IL-6的表达量,收集股骨远端并HE染色,量化骨微细结构相关参数。结果鸢尾素治疗后,糖尿病性骨质疏松模型小鼠体重显著下降,空腹血糖显著降低;血清中鸢尾素水平显著上升,CTX水平显著降低,P1NP水平显著升高;骨组织Runx2 mRNA表达量显著上升,IL-1β、IL-6 mRNA表达量显著降低;原代骨髓间充质干细胞Runx2 mRNA表达量显著上升,IL-1β、IL-6 mRNA表达量显著降低;小鼠单位长度骨小梁表面成骨细胞数量、骨小梁面积百分数、骨小梁宽度均限制增加。结论鸢尾素能够抑制骨组织及间充质干细胞炎症因子的表达,促进成骨分化,改善糖尿病小鼠骨代谢。Objective To investigate the therapeutic effect and mechanism of irisin on diabetic osteoporosis.Methods Diabetic osteoporosis model was established in 6-week-old C57BL/6 mice with intraperitoneal injection of irisin.Body weight,fasting blood glucose,and glucose tolerance tests were conducted.Serum of mice was collected to detect the levels of irisin,CTX,and P1NP.The mRNA expression levels of Runx2,Il-1β,and Il-6 were detected in bone tissue and bone marrow mesenchymal stem cells.The distal femur was collected and stained with HE to quantify the parameters related to bone fine structure.Results After irisin treatment,the diabetic osteoporosis model mice exhibited noteworthy reduction in body weight and fasting blood glucose levels.Additionally,there was a significant rise in irisin concentration in the serum,accompanied by a substantial decrease in CTX concentration and a marked increase in P1NP concentration.Within the bone tissue,the mRNA expression of Runx2 exhibited a pronounced increase,while the mRNA expressions of Il-1βand Il-6 experienced significant decreases.Primary bone marrow mesenchymal stem cells similarly displayed a substantial increase of Runx2 mRNA expression and significant reduction of Il-1βand Il-6 mRNA expression.Furthermore,a noticeable but controlled augmentation was observed in the number of osteoblasts per unit length of bone trabeculae,the percentage of bone trabecular area,and the width of bone trabeculae within the mouse femur.Conclusion Irisin prevents and treats diabetic osteoporosis.The potential mechanism may be to inhibit the expression of inflammatory factors in the bone tissue and mesenchymal stem cells and to promote osteogenic differentiation.

关 键 词：鸢尾素 炎症 成骨分化 糖尿病性骨质疏松 

分 类 号：R274.9[医药卫生—中西医结合]