婴配乳粉中2'-岩藻糖基乳糖和乳糖-N-新四糖含量离子色谱法检测  

Determination of 2′-Fucosylactose and Lacto-N-Neotetraose Contents in Infant Formula by High Performance Anion Exchange Chromatography-Pulsed Amperometric Detector

作　　者：詹胜群 葛城 周荣杰 周钧 ZHAN Sheng-Qun;GE Cheng;ZHOU Rong-Jie;ZHOU Jun(Ausnutria Dairy(China)Co.Ltd.,Changsha 410200,China)

机构地区：[1]澳优乳业(中国)有限公司,长沙410200

出　　处：《应用化学》2024年第3期405-414,共10页Chinese Journal of Applied Chemistry

摘　　要：研究了离子色谱法同时测定婴配乳粉中2′-岩藻糖基乳糖和乳糖-N-新四糖的含量及影响因素。采用离子色谱柱分离,脉冲安培检测器检测和外标法定量分析等方法。通过对不同净化方式、酶解处理和洗脱条件等过程进行考察和优化,比较确定色谱条件和前处理条件对分离的影响,并就线性范围进行探讨。结果表明,优化后的方法线性关系良好,R^(2)>0.999;方法精密度好,相对标准偏差(n=7)<2.7%;方法准确度好,2′-岩藻糖基乳糖回收率为95.97%~103.38%,乳糖-N-新四糖回收率为100.09%~104.01%。该方法能对婴配乳粉中的2′-岩藻糖基乳糖和乳糖-N-新四糖同时测定,操作简单,具有良好的准确度和精密度,可为婴配乳粉质量监控方法提供参考。Simultaneous determination of 2'-fucosylactose and lacto-N-neotetraose in infant formula milk powder by ion chromatography is studied.These 2 human milk oligosaccharides(HMOs)are separated by ion chromatography,and determined by pulse amperometric detector,and quantified using an external standard method.The method is optimized through analyzing different purification methods,enzymatic hydrolysis treatment,different elution conditions,and the linear range.The effects of determining chromatographic conditions and pre-treatment conditions on separation are compared.The results show that the optimized method has a good linear relationship,with R^(2)>0.999.It also has good precision and accuracy,which the relative standard deviation(n=7)is<2.7%,and the recoveries of 2'-fucose lactose and lacto-N-neotetraose are 95.97%~103.38%and 100.09%~104.01%,respectively.This method can simultaneously determine 2'-fucose and lacto-N-neotetraose in infant formula milk powder.It is simple to operate,has good accuracy and precision,and can provide reference for the quality control method of infant formula milk powder.

关 键 词：母乳低聚糖 离子色谱 2′-岩藻糖基乳糖 乳糖-N-新四糖 婴配乳粉 

分 类 号：O657.7[理学—分析化学]