NCG对山羊性腺轴组织中ITGB8、IGFBP3基因相对表达量的影响  

Effects of NCG on the relative expression of ITGB8 and IGFBP3 genes in goat gonadal axis tissue

作　　者：张劲松 韦仕南 骆金红 吴大江 陈祥 ZHANG Jinsong;WEI Shinan;LUO Jinhong;WU Dajiang;CHEN Xiang(Xishui County Agricultural and Rural Bureau,Xishui 564600,China;Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountains Region of Ministry of Education/Key Laboratory of Animal Genetics,Breeding and Reproduction in Guizhou/College of Animal Sciences,Guizhou University,Guiyang 550025,China;Guizhou Institute of Prataculture,Guiyang 550025,China)

机构地区：[1]习水县农业农村局,贵州习水564600 [2]贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州省草业研究所,贵阳550025

出　　处：《黑龙江畜牧兽医》2024年第7期43-48,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(32060753);国家重点研发计划项目(2017YFD0501904);贵州省科技支撑计划项目(黔科合支撑[2020]1Y030号);贵州省肉羊产业技术体系项目(GZRYTX-2023)。

摘　　要：为了探究妊娠早期饲喂N-氨甲酰谷氨酸(N-Carbamylglutamate,NCG)提高山羊产羔数的繁殖机制,试验采用实时荧光定量PCR方法检测了ITGB8、IGFBP3基因在空白组和NCG组(0.15%NCG)卵巢、子宫、输卵管、垂体、下丘脑等性腺轴组织中的相对表达量。结果表明:ITGB8和IGFBP3基因在贵州白山羊5个组织中均有不同程度表达。相同组别不同组织比较,ITGB8基因在空白组的相对表达量为下丘脑>垂体>输卵管>子宫>卵巢,在NCG组的相对表达量为垂体>子宫>下丘脑>输卵管>卵巢;IGFBP3基因在空白组和NCG组中的表达趋势均为子宫>卵巢>输卵管>下丘脑>垂体。相同组织不同组间比较,ITGB8基因在NCG组垂体、卵巢、输卵管、子宫组织中的相对表达量极显著低于空白组(P<0.01),在下丘脑中两组间差异不显著(P>0.05);IGFBP3基因在NCG组垂体、卵巢、输卵管、子宫组织中的相对表达量均极显著低于空白组(P<0.01),在下丘脑中两组间差异不显著(P>0.05)。说明给山羊饲喂NCG能提高ITGB8基因和降低IGFBP3基因的相对表达量,NCG可能通过调控ITGB8和IGFBP3基因的表达影响山羊繁殖性能。In order to investigate the reproductive mechanism of feeding N-carbamylglutamate(NCG)in early gestation to increase lambing number in goats,in the experiment,the relative expression of ITGB8 and IGFBP3 genes in the gonadal tissues of ovary,uterus,oviduct,pituitary gland and hypothalamus in the blank group and NCG group(0.15%NCG)was detected by real-time fluorescence quantitative PCR.The results showed that ITGB8 and IGFBP3 genes were expressed in different degrees in five tissues of Guizhou White goats.Comparing different tissues in the same group,the relative expression of ITGB8 gene in the blank group was hypothalamus>pituitary>oviduct>uterus>ovary;while in the NCG group,the relative expression was pituitary>uterus>hypothalamus>oviduct>ovary.The expression trend of IGFBP3 gene in the blank group and the NCG group was both uterus>ovary>oviduct>hypothalamus>pituitary.Comparing between different groups of the same tissue,the relative expression of ITGB8 gene in the pituitary,ovary,fallopian tube and uterus tissues of the NCG group was extremely significantly lower than that of the blank group(P<0.01),and the difference between the two groups in the hypothalamus was not significant(P>0.05).The relative expression of IGFBP3 gene in the pituitary,ovary,oviduct and uterus tissues of the NCG group was extremely significantly lower than that of the blank group(P<0.01),and the difference between the two groups in the hypothalamus was not significant(P>0.05).The results indicated that feeding NCG to goats could increase the relative expression of ITGB8 gene and decrease IGFBP3 gene,and NCG might affect the reproductive performance of goats by regulating the expression of ITGB8 and IGFBP3 genes.

关 键 词：山羊 NCG ITGB8基因 IGFBP3基因 差异表达量 

分 类 号：S827[农业科学—畜牧学]