PEG-6000模拟干旱胁迫对黄芪属种子萌发的影响  

Effects of PEG-6000 simulated drought stress on seed germination of Astragalus

作　　者：高霞 石凤翎 王涛 郝俊峰 高翠萍 刘思博 GAO Xia;SHI Fengling;WANG Tao;HAO Junfeng;GAO Cuiping;LIU Sibo(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010019,China;Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010010,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,呼和浩特010019 [2]内蒙古自治区农牧业科学院,呼和浩特010010

出　　处：《黑龙江畜牧兽医》2024年第7期97-104,111,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：内蒙古农业大学草原与资源环境学院青年基金项目“黄芪属植物及其杂交种抗旱性比较及黄酮靶向代谢组学分析”;内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目(2022JBGS0040);内蒙古自治区科技重大专项(2021ZD0031)。

摘　　要：为了筛选出抗旱性较强的黄芪属种质材料,试验利用不同浓度(5%、10%、15%、20%)的聚乙二醇(polyethylene,PEG-6000)对6种黄芪(紫花沙打旺、中沙1号沙打旺、春疆1号沙打旺、沙打旺、斜茎黄芪、黄芪草,按1~6编号)的种子进行模拟干旱胁迫发芽试验,以不加PEG-6000的纯化水为对照,测定不同干旱胁迫处理下种子萌发的根长、胚芽长,计算发芽率、发芽势、发芽指数、活力指数、相对发芽率、相对发芽势、相对发芽指数、相对活力指数、根芽比、相对芽长、相对根长11项指标,采用模糊数学隶属函数法进行综合评价。结果表明:不同浓度PEG-6000胁迫下同一品种种子萌发情况不同,1号(紫花沙打旺)在5%、10%和20%浓度PEG-6000胁迫下发芽率、发芽势、发芽指数和活力指数均高于对照,6号(黄芪草)在15%浓度PEG-6000胁迫下发芽率、相对根长和根芽比均高于对照,对种子萌发有促进作用;2号(中沙1号沙打旺)和5号(斜茎黄芪)在各浓度PEG-6000胁迫下发芽势、发芽指数和活力指数均低于对照,种子萌发受到抑制;3号(春疆1号沙打旺)和4号(沙打旺)在各浓度PEG-6000胁迫下发芽势、发芽指数和活力指数与对照均差异不显著(P>0.05)。相同浓度PEG-6000胁迫下不同品种种子萌发情况存在差异,1号(紫花沙打旺)和6号(黄芪草)在各浓度PEG-6000胁迫下发芽率、发芽势、发芽指数和活力指数均较高,二者种子萌发情况优于其他品种,3号(春疆1号沙打旺)发芽率、发芽势、发芽指数和活力指数低、萌发情况最差。6种黄芪抗旱性强弱排序为1号(紫花沙打旺)、6号(黄芪草)、5号(斜茎黄芪)、4号(沙打旺)、2号(中沙1号沙打旺)、3号(春疆1号沙打旺)。说明6种黄芪的抗旱性强弱差异较大,一定浓度的PEG-6000干旱胁迫对不同品种种子萌发的影响不同,其中1号(紫花沙打旺)抗旱性最强,3号(春疆1号沙打旺)抗旱性最弱。In order to screen out the germplasm materials of Astragalus with strong drought resistance,different concentrations(5%,10%,15%,20%)of polyethylene glycol(PEG-6000)were used in this study to simulate drought stress germination test on the seeds of 6 Astragalus(from No.1 to No.6:Astragalus purdomii,A.adsurgens Pall.cv.zhongsha No.1,A.adsurgens Pall.cv.chunjiang No.1,Astragalus adsurgens Pall.,Astragalus laxmannii,Astragalus herb).The purified water without PEG-6000 was used as control.The root length and plumule length of seeds were measured and the germination rate,germination potential,germination index,vitality index,relative germination percentage,relative germination potential,relative germination index,relative vigor index,RL/PL,relative bud length and relative root length were calculated under drought stress treatment.Fuzzy mathematics membership function method was used for comprehensive evaluation.The results showed that the seed germination of the same breed was different under different PEG-6000 concentrations of stress.The germination rate,germination potential,germination index,and vitality index of No.1(Astragalus purdomii)were higher than those in the control when PEG-6000 stress concentrations were 5%,10%,and 20%.The germination rate,relative root length,and RL/PL of No.6(Astragalus herb)were higher than those in the control at a PEG-6000 stress concentration of 15%,which could promote seed germination.The germination potential,germination index,and vitality index of No.2(A.adsurgens Pall.cv.zhongsha No.1)and No.5(Astragalus laxmannii)were lower than those in the control under the various concentrations of stress,and their seed germination was inhibited.There were no significant changes in the germination potential,germination index,and vitality index of No.3(A.adsurgens Pall.cv.chunjiang No.1)and No.4(Astragalus adsurgens Pall.).The seed germination of different breed was different under the same concentration of drought stress.The germination rate,germination potential,germination index,and vital

关 键 词：黄芪属 PEG-6000 干旱胁迫 种子萌发 模糊数学隶属函数法 抗旱性 

分 类 号：S332.4[农业科学—作物遗传育种]