玉女煎对棕榈酸诱导的L细胞损伤的影响  

Effect of Yunvjian on L cell injury induced by palmitic acid

作　　者：王岩松 苏萌 吕洁 雷莉妍 WANG Yansong;SU Meng;LU Jie;LEI Liyan(Shaanxi University of Traditional Chinese Medicine,Shaanxi Provincial Collaborative Innovation Center for Industrialization of Traditional Chinese Medicine Resources,Shaanxi Provincial Key Laboratory of Basic and New Drug Research of Traditional Chinese Medicine,Xianyang 712000,China)

机构地区：[1]陕西中医药大学,陕西省中药资源产业化协同创新中心,陕西省中药基础与新药研究重点实验室,陕西咸阳712000

出　　处：《中国病理生理杂志》2024年第4期706-712,共7页Chinese Journal of Pathophysiology

基　　金：陕西省教育厅重点科学研究计划项目(No.20JY011);陕西省重点研发计划项目(No.2022SF-090)。

摘　　要：目的:研究玉女煎对棕榈酸(PA)诱导的肠L细胞损伤的影响。方法:(1)将不同浓度(1.5、3和6 g/L)玉女煎溶液分别与二苯代苦味肼基自由基(DPPH·)溶液(100 mg/L)混合并避光反应30 min,采用全波长酶标仪检测吸光度的变化,评价玉女煎对DPPH·的清除作用。(2)将小鼠肠内分泌细胞系GLUTag分为空白对照组、PA(400μmol/L)组、PA+玉女煎(1.5 g/L)组、PA+玉女煎(3 g/L)组和PA+玉女煎(6 g/L)组,通过Hoechst 33342细胞核染色实验和MTT实验评价玉女煎对PA诱导的细胞凋亡的影响。(3)将GLUTag细胞分为空白对照组、PA组和PA+玉女煎(6 g/L)组,采用免疫荧光染色检测细胞内胰高血糖素样肽1(GLP-1)表达水平,通过2´,7´-二氯荧光素二乙酸酯(DCFH-DA)荧光探针检测细胞内活性氧(ROS)水平,通过Western blot实验检测超氧化物歧化酶1(SOD1)表达水平。结果:(1)玉女煎在体外可显著清除DPPH·(P<0.01)。(2)与PA组比较,玉女煎能以浓度依赖性方式抑制PA诱导的GLUTag细胞活力降低,在6 g/L时效果最明显(P<0.01)。(3)与PA组比较,6 g/L玉女煎能显著恢复GLUTag细胞的GLP-1(P<0.05)和SOD1表达水平(P<0.01),降低ROS水平(P<0.01)。结论:玉女煎可能通过降低细胞内氧化应激水平抑制PA诱导的GLUTag细胞损伤。ATM:To explore the impact of Yunvjian on palmitic acid(PA)-induced injury of intestinal L cells.METHODS:(1)Various concentrations(1.5,3 and 6 g/L)of Yunvjian solution and 1,1-diphenyl-2-picrylhydrazyl(DPPH·)solution(100 mg/L)were incubated in darkness for 30 min.A full-wavelength microplate reader was employed to measure the absorbance,thereby assessing the scavenging effect of Yunvjian on DPPH·.(2)Mouse intestinal endocrine cell line GLUTag was divided into control group,PA group,PA+Yunvjian(1.5 g/L)group,PA+Yunvjian(3 g/L)group,and PA+Yunvjian(6 g/L)group.The influence of Yunvjian on PA-induced apoptosis was ascertained through Hoechst 33342 nuclear staining and MTT assay.(3)The GLUTag cells were divided into control group,PA group,and PA+Yunvjian(6 g/L)group.The expression of glucagon-like peptide-1(GLP-1)was quantified by immunofluorescence staining.The level of reactive oxygen species(ROS)was determined using 2',7'-dichlorofluorescein diacetate(DCFHDA)fluorescent probe,and superoxide dismutase 1(SOD1)protein level was analyzed by Western blot.RESULTS:(1)Yunvjian exhibited significant DPPH·scavenging activity in vitro(P<0.01).(2)Compared with PA group,Yunvjian markedly attenuated PA-induced decrease in GLUTag cell viability in a concentration-dependent manner,with the most pronounced effect observed at 6 g/L(P<0.01).(3)Compared with PA group,6 g/L Yunvjian significantly enhanced the expression of GLP-1(P<0.05)and SOD1(P<0.01),but reduced ROS level(P<0.01)in GLUTag cells.CONCLUSION:Yunvjian potentially mitigates PA-induced injury in GLUTag cells by diminishing intracellular oxidative stress.

关 键 词：玉女煎 棕榈酸 肠L细胞 氧化应激 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学] R363.2