硝基呋喃抗菌骨水泥的动物体内生物相容性研究  

In vivo biocompatibility studies of nitrofuran antibacterial bone cement

作　　者：郭锐 阚宇辰 高喆 韩庐阳 卜文翰 许杨 储建军 GUO Rui;KAN Yuchen;GAO Zhe;HAN Luyang;BU Wenhan;XU Yang;CHU Jianjun(Hefei Hospital Affiliated to Anhui Medical University,Hefei 230011,China;The Fifth Clinical Medical School of Anhui Medical University,Hefei 230032,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]安徽医科大学附属合肥医院(合肥市第二人民医院)骨科,安徽合肥230011 [2]安徽医科大学第五临床医学院,安徽合肥230032 [3]合肥工业大学药物科学与工程系,安徽合肥230009

出　　处：《安徽医学》2024年第4期403-408,共6页Anhui Medical Journal

基　　金：合肥市关键共性技术研发与重大科技成果工程化项目(编号:2021YL004)。

摘　　要：目的研究硝基呋喃抗菌骨水泥的动物体内生物相容性。方法选用21只C57小鼠,随机分组,注射市售PMMA骨水泥浸提液(浓度分别为40%、80%和100%)、p(10%NFMA-co-MMA)骨水泥浸提液(浓度分别为40%、80%和100%)以及生理盐水(0.9%氯化钠溶液)(7组,n=3)。观察小鼠一般状态及体质量变化;对小鼠肝脏、肾脏进行组织学染色,观察肝肾细胞有无变性、坏死。选用6只新西兰兔进行背部肌肉植入实验(2组,n=3),对样件周围的肌肉组织进行组织学染色,观察样件植入2周和4周后局部组织炎症反应情况。结果所有小鼠未表现出任何不良反应,小鼠均未死亡。所有注射p(10%NFMA-co-MMA)骨水泥浸提液的小鼠相比于注射前体质量均增加,各时间点增量与空白对照组相比较差异无统计学意义(P>0.05)。40%PMMA浸提液组小鼠体质量增加最明显,第三天体质量增加量为(3.56±2.52)g,增量与空白对照组相比较差异无统计学意义(P>0.05);注射p(10%NFMA-co-MMA)骨水泥浸提液的小鼠肝肾细胞未见明显变性、坏死。植入p(10%NFMA-co-MMA)骨水泥后,第2周观察到样件周围组织有大量炎症细胞浸,第4周炎症减轻,结果与PMMA骨水泥植入后相同。结论与临床使用的PMMA骨水泥相比,非浸出抗菌骨水泥p(NFMA-coMMA)在动物体内没有引起明显毒性反应,说明p(NFMA-co-MMA)骨水泥在动物体内具有良好的生物相容性。Objective To study the in vivo biocompatibility of nitrofuran antimicrobial bone cement.Methods Twenty-one C57 mice were randomly divided into groups and injected with commercially available PMMA bone cement extract(concentrations of 40%,80%and 100%),p(10%NFMA-co-MMA)bone cement extract(40%,80%and 100%,respectively)and normal saline(0.9%sodium chloride solu⁃tion)(7 groups,n=3).The changes of general state and body weight of mice were observed.The liver and kidney of mice were stained histologi⁃cally to observe whether liver and kidney cells had degeneration and necrosis.Six New Zealand rabbits were selected for back muscle implanta⁃tion experiments(2 groups,n=3),and the muscle tissues around the samples were histologically stained,and the local tissue reactions were ob⁃served after two weeks and four weeks of implantation.Results All mice did not show any adverse reactions and none of the mice died.All mice injected with p(10%NFMA-co-MMA)bone cement extract had an increased mass compared with the precursor injection,and the in⁃crease was not statistically significant compared with that of the control group(P>0.05).Among them,the body mass increase of mice in the 40%PMMA extract group was the most obvious,and the increase in body mass on the third day was(3.56±2.52)g,and the increase was not sta⁃tistically significant compared with that of the control group(P>0.05).Mouse liver and kidney cells injected with p(10%NFMA-co-MMA)bone cement extract did not show obvious degeneration or necrosis.After implantation of p(10%NFMA-co-MMA)bone cement,a large num⁃ber of inflammatory cell immersion was observed in the surrounding tissues of the sample at week 2,and the inflammation was reduced at week 4,with the same results as after PMMA bone cement implantation.Conclusions Compared with the PMMA bone cement used in clinical prac⁃tice,non-leaching antibacterial bone cement p(NFMA-co-MMA)does not cause significant toxic reactions in animals,indicating that p(NFMA-CO-MMA)bone cement has good biocompatibility in

关 键 词：抗菌 骨水泥 感染 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]