Identification of missing CYP450 enzymes involved in paclitaxel biosynthesis and heterologous reconstitution of baccatin III  

作　　者：DU Jinfa LIAO Pan LU Xu 

机构地区：[1]State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210009,China [2]Department of Biology,Hong Kong Baptist University,Kowloon Tong,Hong Kong,China [3]State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong,Hong Kong,China [4]State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,Beijing 100700,China [5]Medical Botanical Garden,China Pharmaceutical University,Nanjing 210009,China

出　　处：《Chinese Journal of Natural Medicines》2024年第4期291-292,共2页中国天然药物（英文版）

摘　　要：Paclitaxel,a tetracyclic diterpenoid,has garnered attention for its potent anti-cancer properties and intricate molecular structure,making it a significant target for chemical synthesis and biosynthesis[1].However,its natural sources are extremely limited,as it is derived exclusively from the bark of endangered genus Taxus plants,which contain paclitaxel in very low concentrations(0.01%−0.05%)[2-3].Recent advances in synthetic biology present promising opportunities to enhance paclitaxel levels in Taxus cell cultures or to enable the reconstitution of its production in heterologous hosts,such as yeast or tobacco(Nicotiana benthamiana).

关 键 词：Paclitaxel biosynthesis Baccatin III Cytochrome P450 Taxane oxetanase 1 Heterologous reconstitution 

分 类 号：Q812[生物学—生物工程]