Rapid characterization of non-volatile phenolic compounds reveals the reliable chemical markers for authentication of traditional Chinese medicine Xiang-ru among confusing Elsholtzia species  

作　　者：ZENG Zhen ZHANG Chen HU Jiadong WANG Feiyan WU Ziding WANG Jing ZHANG Jun YANG Shuda CHEN Junfeng LI Mingming TONG Qi QIU Shi CHEN Wansheng 

机构地区：[1]The SATCM Key Laboratory for New Resources&Quality Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [2]Department of Pharmacy,Second Affiliated Hospital of Navy Medical University,Shanghai 200003,China [3]Kunming Plant Classification Biotechnology Co.,Ltd.,Kunming 650500,China [4]School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming 650500,China [5]State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,Beijing,100700,China

出　　处：《Chinese Journal of Natural Medicines》2024年第4期375-384,共10页中国天然药物（英文版）

基　　金：supported by the National Key R&D Program of China(No.2022YFC3501700);the Young Elite Scientists Sponsorship Program by Cast(No.2021-QNRC1-02);the Key Project at Central Government Level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302);the Research Project of Science and Technology Commission of Shanghai Municipalit(No.21DZ2202300)。

摘　　要：The aerial parts of Mosla chinensis Maxim.and Mosla chinensis cv.'Jiangxiangru'(MCJ)are widely utilized in traditional Chinese medicine(TCM),known collectively as Xiang-ru.However,due to clinical effectiveness concerns and frequent misidentification,the original plants have increasingly been substituted by various species within the genera Elsholtzia and Mosla.The challenge in distinguishing between these genera arises from their similar morphological and metabolic profiles.To address this issue,our study introduced a rapid method for metabolic characterization,employing high-resolution mass spectrometry-based metabolomics.Through detailed biosynthetic and chemometric analyses,we pinpointed five phenolic compounds—salviaflaside,cynaroside,scutellarein-7-O-D-glucoside,rutin,and vicenin-2—among 203 identified compounds,as reliable chemical markers for distinguishing Xiang-ru from closely related Elsholtzia species.This methodology holds promise for broad application in the analysis of plant aerial parts,especially in verifying the authenticity of aromatic traditional medicinal plants.Our findings underscore the importance of non-volatile compounds as dependable chemical markers in the authentication process of aromatic traditional medicinal plants.

关 键 词：Genus Elsholtzia Genus Mosla Traditional Chinese medicine Xiang-ru Non-volatile phenolic compounds Liquid chromatography-mass spectrometry 

分 类 号：R917[医药卫生—药物分析学]