Circadian disturbance induces erectile dysfunction by impairing endothelial function  

作　　者：Tao Li Yi-Ting Jiang Xin-Zhu Qi Peng Chen Jun-Hao Zhang Fu Luo Jun Qiao Jiang Gu Guang-Shi Du Qiang Wang 

机构地区：[1]Department of Urology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China [2]Department of Urology,Guizhou Provincial People's Hospital,Guiyang 550002,China [3]Department of Otorhinolaryngology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China [4]Guizhou Institute for Food and Drug Control,Guiyang 550004,China [5]Department of Reproductive Center,Guizhou Provincial People's Hospital,Guiyang 550002,China [6]Translational Medicine Research Center of Guizhou Medical University,Guiyang 550025,China

出　　处：《Asian Journal of Andrology》2024年第2期205-211,共7页亚洲男性学杂志（英文版）

基　　金：funded by the National Nature Science Foundation of China(No.82360295 and No.82060276);the Science and Technology Foundation Project of Guizhou Provincial Health Commission(gzwkj2024-150);the Doctor Start-up Fund of Affliated Hospital of Guizhou Medical University(gyfybsky-2023-03);the Science and Technology Department of Guizhou Province(QianKeHeJiChu-ZK[2021]YiBan382).

摘　　要：In order to explore the impact of circadian disturbance on erectile function,we randomly divided 24 adult male rats into groups of control(light on at 8:00 a.m.and off at 8:00 p.m.),dark/dark(DD;constant dark),light/light(LL;constant light),and shift dark/light(DL;light off at 8:00 a.m.and on at 8:0o p.m.).Four weeks later,erectile function was measured and corpora cavernosa were harvested for analysis.The maximum intracavernous pressure(mlcP)and mCP/mean arterial pressure(MAP)ratio in the DD,LL,and DL groups were significantly lower than that in the control group.The LL and DL groups showed significantly attenuated endothelial nitric oxide synthase(eNOS),while DD,LL,and DL showed reduced neuronal nitric oxide synthase(nNOS)at both mRNA and protein levels.The production of nitric oxide(NO)and cyclic guanosinemonophosphate(cGMP)was inhibited by altered light/dark cycles to varying degrees.Circadian disturbance impaired endothelial function and contributed to erectile dysfunction.For the core circadian elements,mRNA expression of circadian locomotor output cycles kaput(Clock)and brain/muscle aryl-hydrocarbon receptor nuclear translocator-like protein 1(Bmal1)was elevated in the DL group,but their protein expression was not significantly changed.DD,LL,and DL increased period 1(Per1)and Per3 levels,while LL and DL increased PER1 levels.No significant difference was found for Per2levels,and PER2 and PER3 concentrations were not significantly changed.Moreover,LL and DL significantly increased cryptochrome-1(CRY1)and CRY2 at both mRNA and protein levels.The altered light/dark rat model showed that circadian disturbance contributed to erectile dysfunction probably by impairing endothelial function.Meanwhile,the core circadian elements were detected in the corpora cavernosa,but these were disrupted.However,which circadian element regulates erectile function and how it works need further analysis.

关 键 词：circadian disturbance endothelial function erectile function light/dark cycle NO/CGMP 

分 类 号：R697[医药卫生—泌尿科学]