The cytosolic isoform of triosephosphate isomerase,ZmTPI4,is required for kernel development and starch synthesis in maize(Zea mays L.)  

作　　者：Wenyu Li Han Wang Qiuyue Xu Long Zhang Yan Wang Yongbiao Yu Xiangkun Guo Zhiwei Zhang Yongbin Dong Yuling Li 

机构地区：[1]State Key Laboratory of Wheat and Maize Crop Science,Collaborative Innovation Center of Henan Grain Crops,College of Agronomy,Henan Agricultural University,Longzi Lake Campus,Zhengzhou 450046,Henan,China

出　　处：《The Crop Journal》2024年第2期401-410,共10页作物学报（英文版）

基　　金：supported by the Major Public Welfare Projects of Henan Province(201300111100 to Yuling Li);Zhongyuan Scholars in Henan Province(22400510003 to Yuling Li);Tackle Program of Agricultural Seed in Henan Province(2022010201 to Yuling Li);Technical System of Maize Industry in Henan Province(HARS-2202-S to Yuling Li);State Key Laboratory of Wheat and Maize Crop Science(SKL2023ZZ05)。

摘　　要：Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and conversion.To understand its function in maize,we characterized a maize TPI mutant,zmtpi4.In comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin content.Protein,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in glycolysis.Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll content.Overexpression of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content.We conclude that ZmTPI4 functions in maize kernel development,starch synthesis,glycolysis,and photosynthesis.

关 键 词：MAIZE Kernel STARCH Weight PHOTOSYNTHESIS 

分 类 号：S513[农业科学—作物学]