Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton  

作　　者：Yujia Duan Xiaoguang Shang Ruiping Tian Weixi Li Xiaohui Song Dayong Zhang Wangzhen Guo 

机构地区：[1]State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [2]Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China

出　　处：《The Crop Journal》2024年第2期411-421,共11页作物学报（英文版）

基　　金：supported by National Key Research and Development Program of China(2022YFD1200300);Jiangsu Key R&D Program(BE2022384);the Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP)(No.10)。

摘　　要：Mitochondrial calcium uniporter(MCU)is a conserved calcium ion(Ca^(2+))transporter in the mitochondrial inner membrane of eukaryotic cells.How MCU proteins regulate Ca^(2+)flow and modulate plant cell development remain largely unclear.Here,we identified the gene GhMCU4 encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton(Gossypium hirsutum).GhMCU4expressed constitutively in various tissues with the higher transcripts in elongating fiber cells.Knockdown of GhMCU4 in cotton significantly elevated the plant height and root length.The calcium signaling pathway was significantly activated and calcium sensor genes,including Ca^(2+)dependent modulator of interactor of constitutively active ROP(GhCMI1),calmodulin like protein(GhCML46),calciumdependent protein kinases(GhCPKs),calcineurin B-like protein(GhCBLs),and CBL-interacting protein kinases(GhCIPKs),were dramatically upregulated in GhMCU4-silenced plants.Metabolic processes were preferentially enriched,and genes related to regulation of transcription were upregulated in GhMCU4-silenced plants.The contents of Ca^(2+)and H_(2)O_(2)were significantly increased in roots and leaves of GhMCU4-silenced plants.Fiber length and Ca^(2+)and H_(2)O_(2)contents in fibers were significantly increased in GhMCU4-silenced plants.This study indicated that GhMCU4 plays a negative role in regulating cell elongation in cotton,thus expanding understanding in the role of MCU proteins in plant growth and development.

关 键 词：Calcium signaling Hydrogen peroxide Metabolic processed Gossypium hirsutum 

分 类 号：S562[农业科学—作物学]