质膜H^(+)-ATPase基因对铝胁迫下水稻硝态氮吸收的 调控作用  

Regulation of Plasma Membrane H^(+)-ATPase Genes on Nitrate Nitrogen Uptake in Rice under Aluminum Stress

作　　者：董雅群 周泽仪 陶光喜[3] 李昆志[2] 周小华 DONG Yaqun;ZHOU Zeyi;TAO Guangxi;LI Kunzhi;ZHOU Xiaohua(College of Agronomy and Life Science,Kunming University,Kunming,Yunnan 650214;Faculty of Life Science and Biotechnology,Kunming University of Science and Technology,Kunming,Yunnan 650093;The Rice Research Institute,Yunnan Agricultural University,Kunming,Yunnan 650100;College of Chemistry and Chemical Engineering,Kunming University,Kunming,Yunnan 650214)

机构地区：[1]昆明学院农学与生命科学学院,云南昆明650214 [2]昆明理工大学生命科学与技术学院,云南昆明650093 [3]云南农业大学水稻研究所,云南昆明650100 [4]昆明学院化学化工学院,云南昆明650214

出　　处：《核农学报》2024年第6期1205-1211,共7页Journal of Nuclear Agricultural Sciences

基　　金：云南省教育厅科学研究基金项目(2023Y0858);国家自然科学基金项目(31760349)。

摘　　要：为探讨细胞质膜ATP酶(PM H^(+)-ATPase)基因对铝胁迫下水稻(Oryza sativa L.)吸收硝态氮(NO_(3)^(-)-N)的调控,本试验以2个水稻品种峰1A和峰1A优5为研究对象,以无铝处理幼苗为对照,分析铝胁迫下水稻根尖PM H^(+)-ATPase活性、H^(+)-泵活性、H^(+)通量、PM H^(+)-ATPase基因家族(OsA1~OsA10)表达水平、PM H^(+)-ATPase和14-3-3蛋白的互作水平以及NO_(3)^(-)-N吸收量。结果表明,与对照相比,铝胁迫下2个水稻品种根尖PM H^(+)-ATPase活性、H^(+)-泵活性和根尖H^(+)通量下降,PM H^(+)-ATPase和14-3-3蛋白的互作水平下降。铝胁迫抑制了OsA1、OsA5、OsA7和OsA8基因的表达,其中OsA7的表达水平显著下调,铝胁迫下峰1A和峰1A优5的OsA7表达量仅为对照组的21%和39%。NO_(3)^(-)-N吸收量较对照下降,分别为对照的81%和85%。综上,PM H^(+)-ATPase基因的表达水平影响水稻对NO_(3)^(-)-N的吸收能力,OsA1、OsA5、OsA7和OsA8在铝胁迫下水稻吸收NO_(3)^(-)-N过程中起关键的调控作用。本研究结果可为增强酸铝条件下水稻吸收NO_(3)^(-)-N的能力,促进水稻生长及增产提供理论依据。To explore the regulation of nitrate nitrogen absorption(NO_(3)^(-)-N)by the cell plasma membrane ATP enzyme(PM H^(+)-ATPase)gene in rice under aluminum(Al)stress,two rice varieties,Feng 1A and Feng 1A super 5,were studied and seedlings without Al treatment were used as control.The activities of PM H^(+)-ATPase hydrolysis and H^(+)-pump,H^(+)efflux,the expression levels of PM H^(+)-ATPase gene(OsA1-OsA10),the level of interaction of PM H^(+)-ATPase and 14-3-3 protein were analysed.The results showed that,the activities of PM H^(+)-ATPase hydrolysis and H^(+)-pump,H^(+)efflux,the level of interaction of PM H^(+)-ATPase and 14-3-3 protein showed a decrease compared with the control.The expression of gene OsA1,OsA5,OsA7 and OsA8 were suppressed.The expression level of OsA7 in Feng 1A and Feng 1A super 5 under Al stress were only 21%and 39%of the control,respectively,and the difference is significant.The uptake of NO_(3)^(-)-N of two rice varieties are 81%and 85%of the control,respectively.In conclusion,the expression level of PM H^(+)-ATPase gene affected the absorption capacity of NO_(3)^(-)-N in rice,OsA1,OsA5,OsA7 and OsA8 could play a key regulatory role in the process of NO_(3)^(-)-N absorption under Al stress.This study provides a theoretical basis for enhancing the ability of rice to absorb NO_(3)^(-)-N under acidic Al conditions,thereby promoting their growth and yield.

关 键 词：水稻 铝胁迫 硝态氮 质膜H+-ATPase QRT-PCR 

分 类 号：S511[农业科学—作物学]