基于Smad3抑制剂SIS3对兔宫颈粘连程度影响的机制研究  

Study on the Mechanism of Smad3 Inhibitor SIS3 on Cervical Adhesion in Rabbits

作　　者：邹倩[1] 易卫兵[2] 杜欣[1] 周利敏[1] 姚冬梅[1] ZOU Qian;YI Wei-bing;DU Xin;ZHOU Li-min;YAO Dong-mei(Department of Gynecology,Maternal and Child Health Hospital of Hubei Province,Wuhan,Hubei 430000,China;Shiyan Centers for Disease Control and prevention,Shiyan,Hubei 430000,Chi-na)

机构地区：[1]湖北省妇幼保健院妇科,湖北武汉430000 [2]十堰市疾病预防控制中心,湖北十堰442000

出　　处：《湖北医药学院学报》2024年第2期115-118,124,共5页Journal of Hubei University of Medicine

基　　金：湖北省卫生健康委员会联合基金项目(WJ2019H274)。

摘　　要：目的:研究信号传导蛋白(Smad3)抑制剂SIS3对宫腔粘连模型兔血清中转化生长因子-β1(TGF-β1)、Smad3表达的影响,为宫腔粘连治疗效果提供理论依据。方法:将18只成年雌性新西兰大白兔随机分为正常组、模型组、SIS3干预组,每组6只。除正常组外,模型组和干预组采用刮宫法建立宫腔粘连模型。造模后干预组每天给予2.5μg/g SIS3注射处理,正常组(开腹未刮宫)和模型组每天给予等体积0.9%氯化钠溶液注射。注射持续14 d后取各组实验兔血清。采用ELISA酶联免疫法检测各组实验兔血清中TGF-β1、Smad3的蛋白表达量。采用实时荧光定量(RT-qPCR)法检测各组实验兔血清中TGF-β1、Smad3的mRNA表达量。结果:与正常组相比,模型组实验兔血清中TGF-β1和Smad3的蛋白及mRNA表达均显著升高(P<0.05);与模型组相比,SIS3干预组实验兔血清中TGF-β1和Smad3的蛋白及mRNA表达显著降低(P<0.05)。此外,TGF-β1蛋白和Smad3蛋白在血清中的表达水平呈显著正相关(R2=0.52),TGF-β1 mRNA和Smad3 mRNA在血清中的表达水平亦呈显著正相关(R2=0.84)。结论:SIS3能抑制宫腔粘连模型兔血清中TGF-β1、Smad3的表达,具有潜在治疗宫腔粘连的作用。Objective To study the effects of SIS3,a specific inhibitor of signal transduction proteins Smad3,on the expressions of transforming growth factor-β1(TGF-β1)and Smad3 in the serum of rabbit model with intrauterine adhesion,and to provide a theoretical basis for the treatment of intrauterine adhesion.Methods Eighteen adult female New Zealand rabbits were randomly divided into control group,model group,and SIS3 treatment group,with 6 rabbits in each group.The rabbits in the model group and SIS3 treatment group underwent uterine curettage to establish the intrauterine adhesion model.After modeling,the rabbits in SIS3 treatment group received daily injections of 2.5μg/g SIS3,while the normal group(open abdomen without curettage)and model group received daily injections of equal volumes of 0.9%saline solution.After 14 days,the blood serum was collected.The protein expression levels of TGF-β1 and Smad3 in the serum of the rabbits were detected using enzyme-linked immunosorbent assay(ELISA).Real-time quantitative PCR was used to analyze the mRNA expressions of serum TGF-β1 and Smad3.Results Compared to the normal group,the protein and mRNA expression levels of TGF-β1 and Smad3 in the serum of rabbits in the model group were significantly increased(P<0.05).Compared to the model group,the protein and mRNA expression levels of TGF-β1 and Smad3 in the serum of rabbits in the SIS3 intervention group were significantly decreased(P<0.05).Additionally,there was a significant positive correlation between the serum protein levels of TGF-β1 and Smad3 in the serum(R^(2)=0.52),as well as between the mRNA levels of TGF-β1 and Smad3 in the serum(R2=0.84).Conclusion SIS3 inhibits the expression of TGF-β1 and Smad3 in the serum of rabbits with intrauterine adhesion models,showing potential therapeutic effects for intrauterine adhesion.

关 键 词：宫腔粘连 转化生长因子-Β1 信号传导蛋白(Smad3) 血清 

分 类 号：R711.74[医药卫生—妇产科学]